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TGFBR3 ELISA Kit
TGFBR3
Reaktivität: Human
Colorimetric
Sandwich ELISA
40-10.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703487
Produktdetails TGFBR3 ELISA Kit
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Target
Alle TGFBR3 ELISA Kits anzeigen
TGFBR3
(Transforming Growth Factor, beta Receptor III (TGFBR3))
Reaktivität
Alle Reaktivitäten für TGFBR3 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
40-10.000 pg/mL
Untere Nachweisgrenze
40 pg/mL
Applikation
ELISA
Verwendungszweck
Human TGF beta R3 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair shows no cross-reactivity with recombinant human (rh) TGF-beta RI, RII, RIIb, TGF-beta 1, beta 2, beta 3, recombinant mouse TGF-beta RIII.
Sensitivität
40 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples20 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu TGFBR3
(ausblenden)
Target
Alle TGFBR3 ELISA Kits anzeigen
TGFBR3
(Transforming Growth Factor, beta Receptor III (TGFBR3))
Andere Bezeichnung
TGF-beta R3 (TGFBR3 Produkte )
Synonyme
TGFBR3 ELISA Kit, DKFZp469I0915 ELISA Kit, BGCAN ELISA Kit, betaglycan ELISA Kit, 1110036H20Rik ELISA Kit, AU015626 ELISA Kit, AW215636 ELISA Kit, TBRIII ELISA Kit, transforming growth factor beta receptor 3 ELISA Kit, transforming growth factor, beta receptor III ELISA Kit, TGFBR3 ELISA Kit, tgfbr3 ELISA Kit, Tgfbr3 ELISA Kit
Hintergrund
Gene Names: TGFBR3 Protein names: Transforming growth factor beta receptor type 3 (TGF-beta receptor type 3) (TGFR-3) (Betaglycan) (Transforming growth factor beta receptor III) (TGF-beta receptor type III)
Gen-ID
7049
UniProt
Q03167
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