APOC3 ELISA Kit

Produktnummer ABIN2702832

Produktdetails APOC3 ELISA Kit

Target: APOC3 (Apolipoprotein C-III (APOC3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 2.5-10.000 pg/mL

Untere Nachweisgrenze: 2.5 pg/mL

Applikation: ELISA

Verwendungszweck: Human ApoC3 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Alpha-2 Microglobulin, ApoA, ApoC1, ApoC2, ApoE, ApoH, Artemin, BMP-6, Calbindin, CK beta 8-1 (CCL23), CNTF, EMMPRIN (CD147), Endostatin, Fibrinogen, FLRG, TNFSF18 (GITR Ligand), GPBB, Hemoglobin, HTRA2, IL-17E, IL-1 R3 (IL1 R Acp), Lactoferrin, Mesothelin, PAPP-A, PD-ECGF (Thymidine Phosphorylase), PDGF R alpha, Pepsinogen 2, Periostin, Persephin, PTH, RAP, SAA (Serum Amyloid A), Serpin F1 (PEDF), Tie-1, TPA, Thrombospondin 2, Ubiquitin+1, Vitronectin, VWF

Sensitivität: 2.5 pg/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples500,000 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Aufbereitung der Proben:

For example, add 2 μL of serum/plasma into a tube with 998.0 μL Assay Diluent to prepare a 500-fold diluted sample. Mix thoroughly and then pipette 2 μL of prepared 500-fold diluted sample into a tube with 1998 μL Assay Diluent to prepare a final 500,000 fold diluted sample.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu APOC3

Target: APOC3 (Apolipoprotein C-III (APOC3))

Andere Bezeichnung: ApoC3 (APOC3 Produkte)

Synonyme: APOC3 ELISA Kit, APOCIII ELISA Kit, HALP2 ELISA Kit, Apoc ELISA Kit, ApoC-III ELISA Kit, apo-CIII ELISA Kit, Apo-CIII ELISA Kit, apolipoprotein C3 ELISA Kit, apolipoprotein C-III ELISA Kit, APOC3 ELISA Kit, Apoc3 ELISA Kit

Hintergrund: Gene Names: APOC3
Protein names: Apolipoprotein C-III (Apo-CIII) (ApoC-III) (Apolipoprotein C3)

Gen-ID: 345

UniProt: P02656

Pathways: Carbohydrate Homeostasis, Lipid Metabolism