CXCL2 ELISA Kit

Produktnummer ABIN2648785

Produktdetails CXCL2 ELISA Kit

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Reaktivität: Maus

Nachweismethode: Colorimetric

Applikation: ELISA

Proben: Serum, Plasma, Cell Culture Cells

Sensitivität: 4 pg/ml

Produktmerkmale: Mouse macrophage inflammatory protein-2 (MIP-2), also known as CXCL2, was originally identified as a heparin-binding protein secreted by an LPS-stimulated mouse macrophage cell line (1). A cDNA clone encoding the protein was isolated from this cell line and characterized (2). Based on its protein and DNA sequences, mouse MIP-2 was classified as a member of the alpha (CXC) chemokine family of inflammatory and immunoregulatory cytokines (3). Mouse MIP-2 cDNA encodes a 100 amino acid residue precursor protein from which the amino-terminal 27 amino acid residues are cleaved to generate the mature mouse MIP-2. The protein sequence of mouse MIP-2 shows approximately 63 % identity to that of mouse KC, another mouse alpha chemokine. Mouse MIP-2 is also 60 % identical to human GROβ and GROγ(2). Based on these protein sequence similarities, it is likely that mouse KC and MIP-2 are homologs of human GROα, β and γ chemokines. Since chemokines with protein sequence homology to human IL-8 have not been identified in mice, it has been suggested that the mouse KC and MIP-2 are functional homologs of human IL-8 in mice (3, 4). A putative mouse homolog of the human IL-8 receptor beta (IL-8 Rβ) has also been cloned. This receptor shows 71 % identity to human IL-8 Rβ and 68 % identity to human IL-8 Rα. Both mouse KC and MIP-2 bind mouse IL-8 Rβ with high affinity (5). Like human IL-8, mouse MIP-2 exhibits potent neutrophil chemotactic activity and may be a key mediator of neutrophil recruitment in response to tissue injury and infection (3, 4). Increased MIP-2 expression has been found to be associated with neutrophil influx in various inflammatory conditions (6 - 10).

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: Mouse Macrophage Inflammatory Protein 2 (MIP-2) ELISA Assay Kit employs quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for MIP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-2 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for MIP-2 is added to the wells and binds to the combination of capture antibody-MIP-2 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of MIP-2 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven MIP-2 standard dilutions and MIP-2 sample concentration determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Andere Bezeichnung: MIP-2 (CXCL2 Produkte)

Synonyme: CINC-2a ELISA Kit, GRO2 ELISA Kit, GROb ELISA Kit, MGSA-b ELISA Kit, MIP-2a ELISA Kit, MIP2 ELISA Kit, MIP2A ELISA Kit, SCYB2 ELISA Kit, GRO3 ELISA Kit, Gro2 ELISA Kit, MIP-2 ELISA Kit, Mgsa-b ELISA Kit, Mip2 ELISA Kit, Scyb ELISA Kit, Scyb2 ELISA Kit, Mip-2 ELISA Kit, GROB ELISA Kit, MIP-2alpha ELISA Kit, C-X-C motif chemokine ligand 2 ELISA Kit, chemokine (C-X-C motif) ligand 2 ELISA Kit, CXCL2 ELISA Kit, Cxcl2 ELISA Kit

Pathways: Cellular Response to Molecule of Bacterial Origin