CXCL2 ELISA Kit

Produktnummer ABIN921067

Maus CXCL2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus CXCL2 und es wurde in 3+ Publikationen zitiert.

Kurzübersicht für CXCL2 ELISA Kit (ABIN921067)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Bindungsspezifität: AA 28-100

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails CXCL2 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: Sandwich Mouse MIP-2 ELISA Kit to quantitate Mouse Cxcl2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: A28-N100

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 4.6%, 7.8%, 4.1%
Inter-Assay Precision (CV%): 5%, 8.5%, 5.6%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für CXCL2 ELISA Kit (ABIN921067)

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Roux, Schaefers, Clark, Weatherholt, Renaud, Scott, LiPuma, Priebe, Gerard, Yoder-Himes: "A putative lateral flagella of the cystic fibrosis pathogen Burkholderia dolosa regulates swimming motility and host cytokine production." in: PLoS ONE, Vol. 13, Issue 1, pp. e0189810, (2018) (PubMed).

Roux, Weatherholt, Clark, Gadjeva, Renaud, Scott, Skurnik, Priebe, Pier, Gerard, Yoder-Himes: "Immune Recognition of the Epidemic Cystic Fibrosis Pathogen Burkholderia dolosa." in: Infection and immunity, Vol. 85, Issue 6, (2017) (PubMed).

Details zu CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Andere Bezeichnung: CXCL2

Hintergrund:

Background: Macrophage Inflammatory Protein 2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 forms homotetramers. MIP2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. MIP2 is chemotactic for polymorphonuclear leukocytes.

Gene Full Name: C-X-C motif chemokine ligand 2

Gen-ID: 20310

UniProt: P10889

Pathways: Cellular Response to Molecule of Bacterial Origin