SERPINA1 ELISA Kit

Produktnummer ABIN2648470

Produktdetails SERPINA1 ELISA Kit

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Reaktivität: Chemical

Nachweismethode: Colorimetric

Applikation: ELISA

Verwendungszweck: Alpha-1 Antitrypsin ELISA Kit allows an easy, rapid and precise quantitative determination of alpha-1-antitrypsin in biological samples.

Proben: Fecal, Serum, Plasma

Analytische Methode: Quantitative

Sensitivität: 1.5 ng/ml

Produktmerkmale: Alpha-1-Antitrypsin is a 52 kD glycoprotein, which is produced by the liver, intestinal macrophages, monocytes and mucous membrane cells of the gut. It belongs to the group of acute phase proteins and is one of the most important proteinase inhibitors. Alpha-1-antitrypsin inhibits, beside others, the proteinases trypsin and the elastase of neutrophils. A lack of α-1-AT leads to an enhanced proteolysis. Only a very small amount of alpha-1-antitrypsin is cleaved or resorbed in the gut. Therefore the measurement of α-1-AT in stool reflects the permeability of the gut during inflammatory processes. Alpha-1 Antitrypsin ELISA Kit allows an easy, rapid and precise quantitative determination of alpha-1-antitrypsin in biological samples. The kit includes all reagents ready to use for preparation of the samples.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Probenmenge: 100 μL

Testdauer: 2 h

Protokoll: The Alpha-1 Antitrypsin ELISA assay determines human alpha-1-antitrypsin according to the "sandwich"-principle. Alpha-1 Antitrypsin in sample, standard and controls binds to antibodies, which are coated to the microtiter plate. After a washing step a peroxidase labeled detection antibody is added. A second washing step is followed by the addition of the substrate which is converted to a colored product by the peroxidase. The reaction is terminated by the addition of an acidic stop solution. The optical densities are read at 450 nm (against the reference wavelength 620 nm) in a microtiter plate reader. The α-1-AT concentration can be calculated from the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Details zu SERPINA1

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Andere Bezeichnung: alpha 1 Antitrypsin (SERPINA1 Produkte)

Synonyme: AACT ELISA Kit, ACT ELISA Kit, GIG25 ELISA Kit, A1AT ELISA Kit, PI ELISA Kit, alpha-1-PI ELISA Kit, A1A ELISA Kit, AAT ELISA Kit, PI1 ELISA Kit, PRO2275 ELISA Kit, alpha1AT ELISA Kit, Pi ELISA Kit, Spi1 ELISA Kit, MGC64429 ELISA Kit, serpina1d ELISA Kit, Aps ELISA Kit, SERPINA3-5 ELISA Kit, serpin family A member 3 ELISA Kit, alpha-1-antiproteinase F precursor ELISA Kit, serpin family A member 1 ELISA Kit, alpha-1-antitrypsin ELISA Kit, calumenin ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 S homeolog ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 ELISA Kit, SH2B adaptor protein 2 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 ELISA Kit, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 L homeolog ELISA Kit, SERPINA3 ELISA Kit, LOC100008973 ELISA Kit, SERPINA1 ELISA Kit, LOC100328621 ELISA Kit, CALU ELISA Kit, Serpina1 ELISA Kit, serpina1.S ELISA Kit, serpina1 ELISA Kit, SPIA5 ELISA Kit, Sh2b2 ELISA Kit, Calu ELISA Kit, serpina1.L ELISA Kit