Albumin ELISA Kit

Produktnummer ABIN2345047

Produktdetails Albumin ELISA Kit

Target: Albumin (ALB)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Serum, Plasma, Urine

Analytische Methode: Quantitative

Sensitivität: 100 pg/mL

Produktmerkmale: Human Albumin ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human albumin in plasma, serum, urine or other biological fluid samples. The kit has a detection sensitivity limit of 5 ng/mL human albumin. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.

Bestandteile:

1. Anti-HSA Antibody Coated Plate : One 96-well strip plate (8 x 12).
2. Biotinylated Anti-HSA Antibody (1000X) : One 20 μL vial.
3. Streptavidin-Enzyme Conjugate : One 20 μL vial.
4. Assay Diluent : One 50 mL bottle.
5. 10X Wash Buffer : One 100 mL bottle.
6. Substrate Solution : One 12 mL amber bottle.
7. Stop Solution (Part. No. 310808): One 12 mL bottle.

Box 2 (shipped on blue ice packs)

Benötigtes Material:

1. Plasma, Serum, Urine or Other Biological Fluids
2. PBS containing 0.1 % BSA
3. 10 μL to 1000 μL adjustable single channel micropipettes with disposable tips
4. 50 μL to 300 μL adjustable multichannel micropipette with disposable tips
5. Multichannel micropipette reservoir
6. Microplate reader capable of reading at 450 nm (620 nm as optional reference wave length)

Anwendungsinformationen

Kommentare:

• Sensitivity limit of 100 pg/mL human albumin
• Detection and quantitation of human albumin in plasma, serum, urine or other biological fluid samples

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

1X Wash Buffer: Dilute the 10X Wash Buffer Concentrate to 1X with deionized water. Stir to homogeneity. Biotinylated Anti-HSA Antibody and Streptavidin-Enzyme Conjugate: Immediately before use dilute the Biotinylated Anti-HSA antibody 1:1000 and the Streptavidin-Enzyme Conjugate 1:2000 with Assay Diluent. Do not store diluted solutions.

Aufbereitung der Proben:

The following recommendations are only guidelines and may be altered to optimize or complement the user's experimental design. Plasma: Collect blood with heparin or EDTA and centrifuge at 4 °C for 10 minutes at 1000 g. Remove the plasma and assay immediately or store samples at -80 °C for up to three months. Normal plasma samples require about 1,000,000 fold dilution with PBS containing 0.1 % BSA immediately before running the ELISA. Prepare a serial dilution of each sample according to Table 2 below, mixing each dilution well before continuing to the next dilution step. Serum: Harvest serum and centrifuge at 4 °C for 10 minutes at 1000 g. Assay immediately or store samples at -80 °C for up to three months. Normal serum samples require about 1,000,000 fold dilution with PBS containing 0.1 % BSA immediately before running the ELISA. Prepare a serial dilution of each sample according to Table 2 below, mixing each dilution well before continuing to the next dilution step. Sample Plasma or Serum PBS with Effective Tubes Sample 0.1 % BSA Dilution 1 5 μL 5 mL 1:1000 2 5 μL of Tube #1 5 mL 1:1,000,000 Table 2. Preparation of 1:1,000,000 dilution of plasma or serum samples. Urine: Harvest urine and centrifuge for 10 minutes at 1000 g at 4 °C. Assay immediately or store samples at -80 °C for up to three months. Normal urine samples require about 1000 fold dilution with PBS containing 0.1 % BSA immediately before running the ELISA. Other Biological Fluids: Centrifuge samples for 10 minutes at 1000 g at 4 °C. Assay immediately or store samples at -80 °C for up to three months.

Testdurchführung:

1. Prepare dilutions of plasma, serum, urine or other biological fluid samples in PBS containing 0.1 % BSA.
2. Add 100 μL of human albumin unknown sample or standard to the Anti-HSA Antibody Coated Plate. Each human albumin unknown sample, standard and blank should be assayed in duplicate.
3. Incubate at 37 °C for at least 2 hours or 4 °C overnight.
4. Wash microwell strips 3 times with 250 μL 1X Wash Buffer per well with thorough aspiration between each wash. After the last wash, empty wells and tap microwell strips on absorbent pad or paper towel to remove excess 1X Wash Buffer.
5. Add 100 μL of the diluted Biotinylated Anti-HSA antibody to each well. Incubate at room temperature for 1 hour on an orbital shaker. 4
6. Wash the strip wells 3 times according to step 4 above.
7. Add 100 μL of the diluted Streptavidin-Enzyme Conjugate to each well. Incubate at room temperature for 1 hour on an orbital shaker.
8. Wash the strip wells 3 times according to step 4 above. Proceed immediately to the next step.
9. Warm Substrate Solution to room temperature. Add 100 L of Substrate Solution to each well, including the blank wells. Incubate at room temperature on an orbital shaker. Actual incubation time may vary from 2-30 minutes. Note: Watch plate carefully, if color changes rapidly, the reaction may need to be stopped sooner to prevent saturation.
10. Stop the enzyme reaction by adding 100 μL of Stop Solution into each well, including the blank wells. Results should be read immediately (color will fade over time).
11. Read absorbance of each microwell on a spectrophotometer using 450 nm as the primary wave length. 5

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Upon receipt, aliquot and store the Biotinylated Anti-HSA Antibody and the HSA Standard at -20°C to avoid multiple freeze/thaw cycles. Store all other components at 4°C.

Referenzen für Albumin ELISA Kit (ABIN2345047)

Yang, Wang, Zhou, Wong, Tian, Hong, Lai, Au, Wei, Liu, Cheng, Liang, Huang, Fan, Zhao, Wang, Ibañez, Luo, Li, Zhong, Chen, Wang, Li, Lai, Qin, Bao, Hutchins, Siu, Huang, Esteban, Tse: "Generation of Human Liver Chimeric Mice with Hepatocytes from Familial Hypercholesterolemia Induced Pluripotent Stem Cells." in: Stem cell reports, Vol. 8, Issue 3, pp. 605-618, (2017) (PubMed).

Bielewicz, Kurzepa, Czekajska-Chehab, Kamieniak, Daniluk, Bartosik-Psujek, Rejdak: "Worse Neurological State During Acute Ischemic Stroke is Associated with a Decrease in Serum Albumin Levels." in: Journal of molecular neuroscience : MN, (2016) (PubMed).

Details zu Albumin

Target: Albumin (ALB)

Andere Bezeichnung: Albumin (ALB Produkte)

Synonyme: PRO0883 ELISA Kit, PRO0903 ELISA Kit, PRO1341 ELISA Kit, ALB ELISA Kit, CSA ELISA Kit, Alb-1 ELISA Kit, Alb1 ELISA Kit, Albza ELISA Kit, LOC100136344 ELISA Kit, alb-a ELISA Kit, alb-b ELISA Kit, albumin ELISA Kit, serum albumin 1 ELISA Kit, albumin S homeolog ELISA Kit, ALB ELISA Kit, Alb ELISA Kit, LOC100136344 ELISA Kit, alb.S ELISA Kit

Hintergrund: Human serum albumin (HSA) is the most abundant protein in human blood plasma. Albumin constitutes about half of the blood serum protein. It is soluble and monomeric. The typical albumin concentration in blood is around 5 g/dL (50 mg/mL). It is produced in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. Albumin transports hormones, fatty acids, and other compounds, buffers pH , and maintains osmotic pressure.

Pathways: Lipid Metabolism