WNT2 ELISA Kit

Produktnummer ABIN2044815

Produktdetails WNT2 ELISA Kit

Target: WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.056 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Wingless Type MMTV Integration Site Family, Member 2 (WNT2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 2 (WNT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 2 (WNT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 2 (WNT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 2 (WNT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu WNT2

Target: WNT2 (Wingless-Type MMTV Integration Site Family Member 2 (WNT2))

Andere Bezeichnung: Wingless Type MMTV Integration Site Family, Member 2 (WNT2) (WNT2 Produkte)

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Pathways: WNT Signalweg