C8B ELISA Kit

Produktnummer ABIN2043207

Produktdetails C8B ELISA Kit

Target: C8B (Complement Component 8, beta Polypeptide (C8B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.31-20 ng/mL

Untere Nachweisgrenze: 0.31 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.139 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Complement Component 8b (C8b) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 8b (C8b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 8b (C8b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 8b (C8b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Component 8b (C8b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu C8B

Target: C8B (Complement Component 8, beta Polypeptide (C8B))

Andere Bezeichnung: Complement Component 8b (C8b) (C8B Produkte)

Synonyme: C82 ELISA Kit, 4930439B20Rik ELISA Kit, AI595927 ELISA Kit, complement C8 beta chain ELISA Kit, complement component 8, beta polypeptide ELISA Kit, C8B ELISA Kit, C8b ELISA Kit, c8b ELISA Kit

Pathways: Komplementsystem