ALDOB ELISA Kit

Produktnummer ABIN2042807

Produktdetails ALDOB ELISA Kit

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.15-10 ng/mL

Untere Nachweisgrenze: 0.15 ng/mL

Applikation: ELISA

Marke: BioAssay™

Sensitivität: < 0.063 ng/mL

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Aldolase B, Fructose Bisphosphate (ALDOB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aldolase B, Fructose Bisphosphate (ALDOB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aldolase B, Fructose Bisphosphate (ALDOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aldolase B, Fructose Bisphosphate (ALDOB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Aldolase B, Fructose Bisphosphate (ALDOB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C

Details zu ALDOB

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Andere Bezeichnung: Aldolase B, Fructose Bisphosphate (ALDOB) (ALDOB Produkte)

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