ALDOB ELISA Kit

Produktnummer ABIN5674805

Produktdetails ALDOB ELISA Kit

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum

Analytische Methode: Quantitative

Sensitivität: 0057 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu ALDOB

Target: ALDOB (Aldolase B, Fructose-Bisphosphate (ALDOB))

Andere Bezeichnung: Aldolase B, Fructose Bisphosphate (ALDOB) (ALDOB Produkte)

Synonyme: aldb ELISA Kit, aldo2 ELISA Kit, xaldb ELISA Kit, ALDOB ELISA Kit, aldob ELISA Kit, ALDB ELISA Kit, ALDO2 ELISA Kit, Aldo-2 ELISA Kit, Aldo2 ELISA Kit, BC016435 ELISA Kit, LIV10 ELISA Kit, fb25f09 ELISA Kit, wu:fb25f09 ELISA Kit, aldolase B ELISA Kit, aldolase, fructose-bisphosphate B ELISA Kit, aldolase, fructose-bisphosphate B S homeolog ELISA Kit, aldolase b, fructose-bisphosphate ELISA Kit, aldolase B, fructose-bisphosphate ELISA Kit, xaldb ELISA Kit, aldob ELISA Kit, aldob.S ELISA Kit, ALDOB ELISA Kit, Aldob ELISA Kit

Hintergrund: Alternative name: ALDO-BL, Liver-type aldolase

Gen-ID: 230163

UniProt: Q91Y97