FCGR2B ELISA Kit

Produktnummer ABIN1889450

Produktdetails FCGR2B ELISA Kit

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Bindungsspezifität: AA 46-217

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 46.9-3000 pg/mL

Untere Nachweisgrenze: 46.9 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD32/FCGR2b/c

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: A46-P217

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <10pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A46-P217

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Kommentare:

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Tissue Specificity: Isoform IIC1 is detected in monocytes, macrophages, polymorphonuclear cells and natural killer cells.

Plattentyp: Pre-coated

Protokoll: human CD32 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD32 has been precoated onto 96-well plates. Standards(NSO, A46-P217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD32 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD32 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL human CD32 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CD32 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 147, Standard deviation: 6.62, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 890, Standard deviation: 32.93, CV(%): 3.7
• Sample 3: n=16, Mean(pg/ml): 1865, Standard deviation: 80.2, CV(%): 4.3,
• Sample 1: n=24, Mean(pg/ml): 168, Standard deviation: 9.75, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 904, Standard deviation: 41.6, CV(%): 4.6
• Sample 3: n=24, Mean(pg/ml): 2162, Standard deviation: 112.42, CV(%): 5.2

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Details zu FCGR2B

Target: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Andere Bezeichnung: FCGR2B (FCGR2B Produkte)

Synonyme: CD32 ELISA Kit, CD32B ELISA Kit, FCG2 ELISA Kit, FCGR2 ELISA Kit, IGFR2 ELISA Kit, FCGR2B ELISA Kit, AI528646 ELISA Kit, F630109E10Rik ELISA Kit, Fc[g]RII ELISA Kit, FcgRII ELISA Kit, Fcgr2 ELISA Kit, Fcgr2a ELISA Kit, Fcr-2 ELISA Kit, Fcr-3 ELISA Kit, Ly-17 ELISA Kit, Ly-m20 ELISA Kit, LyM-1 ELISA Kit, Lym-1 ELISA Kit, FCRII ELISA Kit, FcRII ELISA Kit, Fc fragment of IgG receptor IIb ELISA Kit, Fc fragment of IgG, low affinity IIb, receptor (CD32) ELISA Kit, Fc receptor, IgG, low affinity IIb ELISA Kit, low affinity immunoglobulin gamma Fc region receptor II ELISA Kit, FCGR2B ELISA Kit, Fcgr2b ELISA Kit, LOC100856270 ELISA Kit

Hintergrund:

Protein Function: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells.

Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Synonyms: Low affinity immunoglobulin gamma Fc region receptor II-c,IgG Fc receptor II-c,CDw32,Fc-gamma RII-c,Fc-gamma-RIIc,FcRII-c,CD32,FCGR2C,CD32, FCG2, IGFR2,

Full Gene Name: Low affinity immunoglobulin gamma Fc region receptor II-c

Cellular Localisation: Isoform IIC4: Cytoplasm.

Gen-ID: 2213, 9103

UniProt: P31995

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling