BAFF ELISA Kit

Produktnummer ABIN1112559

Produktdetails BAFF ELISA Kit

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5-4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Verwendungszweck: For quantitative detection of BAFF in serum, plasma, body fluids, tissue lysates or cell culture supernates.

Proben: Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Sensitivität: < 2 pg/mL

Bestandteile: 1. One 96-well plate pre-coated with anti-Human BAFF antibody 2. Lyophilized BAFF standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human BAFF antibody (Concentrated): 130 µl.

Benötigtes Material: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Anwendungsinformationen

Kommentare:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BAFF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BAFF polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the BAFF amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of BAFF can be calculated.

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Aufbereitung der Proben:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate or body fluids, cell culture supernate: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin or EDTA as the anticoagulant. Centrifuge for 15min at 2-8° C at 1000 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate can not be used as anticoagulant here. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Details zu BAFF

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Andere Bezeichnung: BAFF (TNFSF13B Produkte)

Synonyme: BAFF ELISA Kit, BLYS ELISA Kit, CD257 ELISA Kit, DTL ELISA Kit, TALL-1 ELISA Kit, TALL1 ELISA Kit, THANK ELISA Kit, TNFSF20 ELISA Kit, ZTNF4 ELISA Kit, BAFF-R ELISA Kit, BAFFR ELISA Kit, BROMIX ELISA Kit, CD268 ELISA Kit, CVID4 ELISA Kit, prolixin ELISA Kit, TNFSF13B ELISA Kit, RGD1561519 ELISA Kit, RGD1560810 ELISA Kit, BLyS ELISA Kit, D8Ertd387e ELISA Kit, zTNF4 ELISA Kit, 2010006P15Rik ELISA Kit, Baffr ELISA Kit, Bcmd ELISA Kit, Bcmd-1 ELISA Kit, Bcmd1 ELISA Kit, Lvis22 ELISA Kit, zgc:172115 ELISA Kit, TNF superfamily member 13b ELISA Kit, TNF receptor superfamily member 13C ELISA Kit, tumor necrosis factor (ligand) superfamily, member 13b ELISA Kit, tumor necrosis factor receptor superfamily, member 13c ELISA Kit, tumor necrosis factor superfamily member 13b ELISA Kit, TNFSF13B ELISA Kit, TNFRSF13C ELISA Kit, Tnfsf13b ELISA Kit, Tnfrsf13c ELISA Kit, tnfsf13b ELISA Kit

Hintergrund: B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It was expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.As an immunostimulant, BAFF is necessary for maintaining normal immunity.

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response