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1 | ABIN198075 | GS IP ELISA WB | Rabbit | IgG | C-Term | Anmelden zum Anzeigen | Polyclonal | 0 | ||
1 | ABIN198076 | IP ELISA WB | Mouse | IgG2a, lambda | C-Term | Anmelden zum Anzeigen | 0 | |||
1 | ABIN218719 | IP WB | Rabbit | IgG | AA 776-786 | Anmelden zum Anzeigen | Polyclonal | 0 | ||
1 | ABIN1938710 | IF IP IHC (p) WB | Rabbit | IgG | AA 737-787 | Anmelden zum Anzeigen | Polyclonal | 0 |
General |
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Antigen | Signal Transducer and Activator of Transcription 5B (STAT5B) Antikörper |
Epitop | AA 774-787 Alternativen |
Reaktivität | Rind (Kuh), Hund, Hamster, Pferd, Human, Maus, Schwein, Ratte (Rattus) Alternativen |
Wirt | Kaninchen Alternativen |
Klonalität | |
Konjugat | Dieser STAT5B Antikörper ist unkonjugiert Alternativen |
Applikation |
Immunoprecipitation (IP), Western Blotting (WB)
Alternativen
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Hersteller | Anmelden zum Anzeigen |
Produktdetails anti-STAT5B AntikörperTarget Details STAT5B Anwendungsinformationen Handhabung Bilder |
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Spezifität | Recognizes the 90-92kD forms of STAT5B protein. Species cross-reactivity: Human and mouse. |
Homologie | Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%) Panda, Bat, Chicken, Xenopus (93%) Opossum (86%). |
Reinigung | Protein A purified |
Immunogen | Amino acids 774-787 of human STAT5B (GRPMDSQWIPHAQS). The peptide was coupled to KLH prior to immunization. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Horse, Pig (100%), Panda, Bat, Chicken, Xenopus (93%), Opossum (86%). |
Isotyp | IgG |
Target Details STAT5BProduktdetails anti-STAT5B Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben |
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Antigen | |
Andere Bezeichnung | STAT5B / STAT (STAT5B Antibody Abstract) |
Hintergrund |
Name/Gene ID: STAT5B Synonyms: STAT5B, Transcription factor STAT5B, STAT5 |
Gen-ID | 6777 |
UniProt | P51692 |
Pathways | JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation |
AnwendungsinformationenProduktdetails anti-STAT5B Antikörper Target Details STAT5B Handhabung Bilder zurück nach oben |
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Applikationshinweise |
Approved: IP, WB Usage: Western Blot: 0.5-2 mg/mL detects STAT5B (doublet of 90kD and 92kD) in. human A431 and mouse 3T3 fibroblasts using enhanced chemiluminescence with a five minute exposure. Immunoprecipitation: 4 μg immunoprecipitates STAT5B from A431 carcinoma cells and a. previous lot immunoprecipitated STAT5B from human Raji cells. A RIPA lysate of human A431 cells (500 μg protein) was incubated with 4 μg of anti-STAT 5B Immune complexes were precipitated with protein A agarose and analyzed by immunoblotting with anti- STAT 5B. Bands were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP in conjunction with enhanced chemiluminescence detection. Positive Antigen Control: mouse 3T3 cell lysate. Add 2.5 μL of 2-mercaptoethanol. per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails anti-STAT5B Antikörper Target Details STAT5B Anwendungsinformationen Bilder zurück nach oben |
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Format | Liquid |
Konzentration | Lot specific |
Buffer | Supplied as a liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide. |
Konservierungsmittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Lagerung | 4 °C,-20 °C |
Informationen zur Lagerung |
Long term: Add glycerol (40-50%) -20°C Short term: +4°C |