STAT5B Antikörper (N-Term)

Produktnummer ABIN6744443

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch STAT5B in WB, IHC und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Hund, Pferd, Schwein, Kaninchen und Huhn.

Kurzübersicht für STAT5B Antikörper (N-Term) (ABIN6744443)

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Schwein, Kaninchen, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STAT5B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-STAT5B Antikörper

Bindungsspezifität: N-Term

Spezifität: Human STAT5b

Homologie: Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig, Chicken (100%) Bovine (91%) Xenopus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human STAT5B (P51692, NP_036580). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Galago, Mouse, Rat, Elephant, Panda, Dog, Rabbit, Horse, Pig, Opossum, Zebra finch, Chicken (100%), Bovine, Bat, Guinea pig, Xenopus (92%), Monkey, Sheep, Hamster, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 μL sterile ddH2O.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu STAT5B

Target: STAT5B (Signal Transducer and Activator of Transcription 5B (STAT5B))

Andere Bezeichnung: STAT5B / STAT

Hintergrund: Name/Gene ID: STAT5B

Synonyms: STAT5B, Transcription factor STAT5B, STAT5

Gen-ID: 6777

NCBI Accession: NP_036580

UniProt: P51692

Pathways: JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation