Llama IgG isotype control (FITC)
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- Target Alle IgG Produkte
- IgG
- Reaktivität
- Llama
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Wirt
- Llama
- Konjugat
- FITC
- Applikation
- Flow Cytometry (FACS), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Aufreinigung
- Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.
- Isotyp
- IgG
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- Applikationshinweise
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Flow Cytometry Dilution: User Optimized
Application Note: Llama IgG2 FITC conjugated isotype control can be utilized in Flow Cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting experiments where determination of sample isotype is important.
Western Blot Dilution: User Optimized
FLISA Dilution: User Optimized
IF Microscopy Dilution: User Optimized
- Kommentare
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Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
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