Human IgG isotype control (FITC)

Details zu Produkt Nr. ABIN398849, Anbieter: Anmelden zum Anzeigen
Isotyp
Wirt
Human
183
178
175
141
134
116
86
67
67
66
66
61
53
51
38
32
24
16
15
11
7
4
3
Konjugat
FITC
169
153
150
107
106
50
41
41
24
22
13
12
12
11
9
8
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
Blocking Reagent (BR), Isotype Control (IsoC)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Isotyp IgG
Produktmerkmale Purified Human IgG (Whole Molecule) Control [FITC] is highly purified from normal human serum through Protein G chromatography. It is suitable to be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunoblotting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reaction.
Absorption Wavelength: 492 nm,
Emission Wavelength: 518 nm,
Fluorochrome/Protein Ratio: 4.1 moles FITC per mole of IgG
Reinigung Protein G chromatography
Applikationshinweise Each Investigator should determine their own optimal working dilution for specific applications.
Kommentare

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Konzentration 0.5 mg/mL
Buffer PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freezing and thawing.
Product is photosensitive and should be protected from light.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
Produkt verwendet in: Tomita, Fuchimoto, Mori, Kato, Uemura, Handa, Tazawa, Ohdan, Okamoto, Kuroda: "Production of anti-ABO blood group antibodies after minor ABO-incompatible bone marrow transplantation in NOD/SCID/gamma(c)(null) mice." in: Clinical transplantation, Vol. 27, Issue 6, pp. E702-8, 2013 (PubMed).

Lim, Kim, Aref, Kamm, Raghunath: "Complementary effects of ciclopirox olamine, a prolyl hydroxylase inhibitor and sphingosine 1-phosphate on fibroblasts and endothelial cells in driving capillary sprouting." in: Integrative biology : quantitative biosciences from nano to macro, Vol. 5, Issue 12, pp. 1474-84, 2013 (PubMed).