anti-Human MTOR Antikörper für Immunofluorescence

Recommended MTOR Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen

Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
Synonyme für dieses Antigen anzeigen

FRAP1
FRAP
FRAP2
RAFT1
RAPT1
2610315D21Rik
AI327068
Frap1
flat
frap1
tor
wu:fc22h08
mechanistic target of rapamycin kinase
mechanistic target of rapamycin
mechanistic target of rapamycin (serine/threonine kinase)
MTOR
Mtor
mtor

Epitop

AA 200-250
Alternativen

Reaktivität

Human, Ratte (Rattus)
Alternativen

421

229

219

Wirt

Kaninchen
Alternativen

368

Klonalität

Konjugat

Dieser MTOR Antikörper ist unkonjugiert
Alternativen

Applikation

Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Proximity Ligation Assay (PLA), Western Blotting (WB)
Alternativen

307

162

138

Hersteller

Anmelden zum Anzeigen

Hersteller Produkt- Nr.

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4 Publikationen vorhanden

Bilder

Immunofluorescence (IF) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 200-250) antibody (ABIN151707)

Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 200-250) antibody (ABIN151707)

Proximity Ligation Assay (PLA) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 200-250) antibody (ABIN151707)

Produktnummer ABIN151707

Preis und Verfügbarkeit auf Anfrage.

Menge

Preis

100 μL

$556.31

Product detail page

Relevance Score	ABIN	Application	Host	Isotype	Epitope	Hersteller	Clonality	References
11.340238	ABIN2855088	ChIP ICC IF IP IHC (p) WB	Rabbit	IgG	C-Term	Anmelden zum Anzeigen	Polyclonal	0
11.340238	ABIN2705013	IC IF WB	Rabbit		C-Term	Anmelden zum Anzeigen	Polyclonal	0
11.340238	ABIN1531447	IF IHC ELISA	Rabbit	IgG	AA 2447-2496, pSer2481	Anmelden zum Anzeigen	Polyclonal	0
11.340238	ABIN3031742	IF IHC ELISA WB	Rabbit	Ig Fraction	AA 2459-2488	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN390217	IF IHC (p) WB	Rabbit	Ig Fraction	AA 2459-2488, Ser2481	Anmelden zum Anzeigen	Polyclonal	1
1	ABIN196962	IF IHC (p) WB	Rabbit		pSer2448	Anmelden zum Anzeigen	Polyclonal	4
1	ABIN447030	ICC IF IHC (p) IHC WB	Rabbit			Anmelden zum Anzeigen	Polyclonal	1
1	ABIN2755575	IC IF IP IHC WB	Rabbit		C-Term, pSer2448	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN319329	IF IHC (p) WB	Rabbit		Ser2448	Anmelden zum Anzeigen	Polyclonal	3
1	ABIN6282465	ELISA IF IHC (p) WB	Rabbit	IgG	AA 2390-2470, pSer2448	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN2879300	IF IHC IHC (p) WB	Rabbit		Ser2442	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN1870445	IF IHC WB	Rabbit	IgG	pSer2448	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN214561	ELISA IF IHC IHC (p) WB	Rabbit		AA 2440-2457	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN4904450	IF IHC WB	Rabbit	IgG		Anmelden zum Anzeigen	Polyclonal	0
1	ABIN1737123	IF IHC (p) ELISA WB	Rabbit	IgG	AA 2440-2457	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN5961011	ELISA IF IHC (p) WB	Rabbit	IgG	pSer2481	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN5961014	ELISA IF IHC (p) WB	Rabbit	IgG	Ser2448	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN5961015	ELISA IF IHC (p)	Rabbit	IgG	Thr2446	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN5961009	ELISA IF IHC (p) WB	Rabbit	IgG	pSer2448	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN303334	EIA IF IHC (p) WB	Rabbit		AA 2440-2457	Anmelden zum Anzeigen	Polyclonal	0

Weitere Produktkategorien zu MTOR Antikörper

anti-MTOR Antikörper für Immunofluorescence mit den meisten Publikationen

Human Polyclonal MTOR Primary Antibody für ICC, IF - ABIN151707 (4 Publications): Bolster, Vary, Kimball, Jefferson: Leucine regulates translation initiation in rat skeletal muscle via enhanced eIF4G phosphorylation. in The Journal of nutrition 2004 (PubMed)
Human Polyclonal MTOR Primary Antibody für IF, IHC - ABIN362262 (3 Publications): Albanell, Dalmases, Rovira, Rojo: mTOR signalling in human cancer. in Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2007 (PubMed)
Human Polyclonal MTOR Primary Antibody für IF, IHC (p) - ABIN390217 (1 Publications): Cao, Dong, Meng, Liu, Liao, Liu: MiR-511 inhibits growth and metastasis of human hepatocellular carcinoma cells by targeting PIK3R3. in Tumour biology 2015 (PubMed)
Human Polyclonal MTOR Primary Antibody für ICC, IF - ABIN447030 (1 Publications): Bennett, Stockley, Fleming, Mandal, OPrey, Ryan, Robson, Leung: Does androgen-ablation therapy (AAT) associated autophagy have a pro-survival effect in LNCaP human prostate cancer cells? in BJU international 2013 (PubMed)

General
Antigen	Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antikörper
Epitop	AA 200-250 Alternativen
	(69), (32), (30), (23), (21), (18), (18), (15), (15), (12), (8), (7), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität	Human, Ratte (Rattus) Alternativen
	(421), (229), (219), (16), (8), (6), (6), (6), (6), (5), (4), (3), (2), (1), (1), (1)
Wirt	Kaninchen Alternativen
	(368), (49), (3), (1)
Klonalität	Polyklonal
Konjugat	Dieser MTOR Antikörper ist unkonjugiert Alternativen
	(10), (10), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation	Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Proximity Ligation Assay (PLA), Western Blotting (WB) Alternativen (307), (162), (138), (80), (63), (52), (34), (19), (16), (8), (7), (7), (3), (2), (2), (2), (2), (1), (1)
	4 Publikationen vorhanden
Hersteller	Anmelden zum Anzeigen
Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN151707) Bilder
Reinigung	Immunogen affinity purified
Immunogen	The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Mammalian Target of Rapamycin using the numbering given in entry NP_004949.1 (GeneID 2475).
Plasmids, Primers & others
Target Details MTOR Produktdetails anti-MTOR Antikörper Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN151707) Bilder zurück nach oben
Antigen
Andere Bezeichnung	MTOR / FRAP1 (FRAP1 Antibody Abstract)
Hintergrund	Gene Symbol: MTOR
Gen-ID	2475
NCBI Accession	NP_004949
Pathways	PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events
Anwendungsinformationen Produktdetails anti-MTOR Antikörper Target Details MTOR Handhabung Referenzen für anti-MTOR Antikörper (ABIN151707) Bilder zurück nach oben
Applikations-hinweise	Western Blot 1:2000 - 1:10000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 2- 10 μg/mg lysate, Proximity Ligation Assay 1:1000-1:20000
Kommentare	The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Beschränkungen	Nur für Forschungszwecke einsetzbar
Handhabung Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Referenzen für anti-MTOR Antikörper (ABIN151707) Bilder zurück nach oben
Format	Liquid
Konzentration	1.0 mg/mL
Buffer	Tris-Citrate/Phosphate ( pH 7.0 - 8.0) Buffer contains: 0.09 % Sodium Azide
Konservierungs-mittel	Sodium azide
Vorsichtsmaßnahmen	This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung	Do not freeze.
Lagerung	4 °C
Informationen zur Lagerung	Store at 4°C. Do not freeze.
Referenzen für anti-MTOR Antikörper (ABIN151707) Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:	Suzuki, Roa, Kawamoto, Ishige, Wistuba, Li, Thomas, Shoda: "Expression of insulin-like growth factor I receptor as a biomarker for predicting prognosis in biliary tract cancer patients." in: Molecular and clinical oncology, Vol. 3, Issue 3, pp. 464-470, 2015 Weitere Details: Immunohistochemistry Liu, Yang: "MTDH and EphA7 are markers for metastasis and poor prognosis of gallbladder adenocarcinoma." in: Diagnostic cytopathology, Vol. 41, Issue 3, pp. 199-205, 2013 (Probematerial (Species): Human). Weitere Details: Immunohistochemistry Lionello, Blandamura, Loreggian, Ottaviano, Giacomelli, Marchese-Ragona, Velardita, Staffieri, Marioni: "High mTOR expression is associated with a worse oncological outcome in laryngeal carcinoma treated with postoperative radiotherapy: a pilot study." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 41, Issue 2, pp. 136-40, 2012 Bolster, Vary, Kimball, Jefferson: "Leucine regulates translation initiation in rat skeletal muscle via enhanced eIF4G phosphorylation." in: The Journal of nutrition, Vol. 134, Issue 7, pp. 1704-10, 2004
Bilder Produktdetails anti-MTOR Antikörper Target Details MTOR Anwendungsinformationen Handhabung Referenzen für anti-MTOR Antikörper (ABIN151707) zurück nach oben
Bilder des Herstellers	Immunofluorescence detection of mTor (red), using ABIN151707: Sample: L6 myotubes obs... Immunoprecipitation of mTOR for use in Kinase Assays. Sample: Recombinant 4E-BP1 was... Western Blot: TOR/mTOR Antibody [ABIN151707] - Whole cell lysate from HeLa (5, 15 and... Western Blot: TOR/mTOR Antibody [ABIN151707] - Whole cell lysate (50, 15, 5 ug) from ... Proximity Ligation Assay: TOR/mTOR Antibody [ABIN151707] - Secondary-conjugate Duolin... Western Blot: TOR/mTOR Antibody - Detection of Human mTOR by Western Blot. Samples: ... Western Blot: TOR/mTOR Antibody - Whole cell lysate (50 ug) from HeLa, 293T, and Jur... Images for product: anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 200-250) antibody Immunofluorescence detection of mTor (red), using ABIN151707: Sample: L6 myotubes observed by confocal fluorescent microspopy. Immunoprecipitation of mTOR for use in Kinase Assays. Sample: Recombinant 4E-BP1 was incubated at 30 degrees C with mTOR immunoprecipitate and 32P-gamma ATP. ABIN151707 was used at a dilution of 2 mcg/ml lysate. Western Blot: TOR/mTOR Antibody [ABIN151707] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti-mTOR antibody used for WB at 0.4 ug/ml (A) used for IP at 3 ug/mg lysate. mTOR was also immunoprecipitated by a previous lot of this antibody and by rabbit anti-mTOR antibodies ABIN151707 and ABIN1517071. For blotting immunoprecipitated mTOR, ABIN1517071 was used at 1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B). Western Blot: TOR/mTOR Antibody [ABIN151707] - Whole cell lysate (50, 15, 5 ug) from HeLa, and (50 ug) 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Proximity Ligation Assay: TOR/mTOR Antibody [ABIN151707] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RPL26 (NBP2-22233) and rabbit anti-human mTOR (ABIN151707). Image merged from DAPI (2ms) and Texas Red (100ms) exposures, 40X magnification. Western Blot: TOR/mTOR Antibody - Detection of Human mTOR by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa, and (50 ug) 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Western Blot: TOR/mTOR Antibody - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.