anti-Xenopus laevis MAPK3 Antikörper für Western Blotting

Recommended MAPK3 Antibody

Antigen
Mitogen-Activated Protein Kinase 3 (MAPK3) Antikörper
  • ERK-1
  • ERK1
  • ERT2
  • HS44KDAP
  • HUMKER1A
  • P44ERK1
  • P44MAPK
  • PRKM3
  • p44-ERK1
  • p44-MAPK
  • Erk-1
  • Erk1
  • Ert2
  • Esrk1
  • Mnk1
  • Mtap2k
  • Prkm3
  • p44
  • p44erk1
  • p44mapk
  • fi06b09
  • wu:fi06b09
  • zERK1
  • Tb08.10J17.940
  • MAPK1
  • MNK1
  • ATMAPK3
  • ATMPK3
  • T6D9.4
  • mitogen-activated protein kinase 3
  • mitogen-activated protein kinase 3
  • mitogen activated protein kinase 3
  • mitogen-activated serine/threonine-protein kinase
  • MAPK3
  • Mapk3
  • mapk3
  • Tc00.1047053509475.10
  • Tb927.8.3550
  • CEK1
  • MPK3
Reaktivität
Huhn, Rind (Kuh), Fruchtfliege (Drosophila melanogaster), Human, Maus, Ratte (Rattus), Schaf, Xenopus laevis
549
312
288
50
40
37
37
23
19
18
18
10
10
9
9
8
7
4
3
2
2
2
1
1
1
1
Wirt
Kaninchen
434
119
19
6
1
Klonalität
Polyklonal
Konjugat
Dieser MAPK3 Antikörper ist konjugiert mit Atto 633
35
26
25
15
14
14
9
9
9
9
9
9
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
439
206
199
88
75
64
56
39
37
35
15
13
8
4
3
2
1
1
1
Optionen

Bilder

Produktnummer ABIN2486608
$ 458.90
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Clonality References Details
32.45012 ABIN2486605 ICC FACS IF IHC WB Atto 488 Rabbit Polyclonal 3
32.45012 ABIN2486607 ICC FACS IF IHC WB Atto 594 Rabbit Polyclonal 3
32.45012 ABIN2486924 ICC FACS IF IHC WB PerCP Rabbit Polyclonal 3
32.45012 ABIN2486604 ICC FACS IF IHC WB Atto 390 Rabbit Polyclonal 3
32.45012 ABIN2486606 ICC FACS IF IHC WB Atto 565 Rabbit Polyclonal 3
32.45012 ABIN2486609 ICC FACS IF IHC WB Atto 655 Rabbit Polyclonal 3
32.45012 ABIN2486610 ICC FACS IF IHC WB Atto 680 Rabbit Polyclonal 3
32.45012 ABIN2486611 ICC FACS IF IHC WB Atto 700 Rabbit Polyclonal 3
32.45012 ABIN2486919 ICC FACS IF IHC WB APC Rabbit Polyclonal 3
32.45012 ABIN2486923 ICC FACS IF IHC WB PE-Atto 594 Rabbit Polyclonal 3
32.45012 ABIN2486921 ICC FACS IF IHC WB FITC Rabbit Polyclonal 3
32.45012 ABIN2486920 ICC FACS IF IHC WB Biotin Rabbit Polyclonal 3
32.45012 ABIN2486926 ICC FACS IF IHC WB Streptavidin Rabbit Polyclonal 3
32.45012 ABIN2486922 ICC FACS IF IHC WB HRP Rabbit Polyclonal 3
32.45012 ABIN2486925 ICC FACS IF IHC WB PE Rabbit Polyclonal 3
32.45012 ABIN2486918 ICC FACS IF IHC WB Alkaline Phosphatase (AP) Rabbit Polyclonal 3
21.950119 ABIN361833 ICC FACS IF IHC WB Rabbit Polyclonal 9
5.5 ABIN5551547 IF IHC (fro) WB Rabbit IgG Tyr204, pThr202 Polyclonal 1
4 ABIN265088 DB WB Rabbit IgG pThr202, pTyr204 Polyclonal 1
4 ABIN229080 IHC WB Rabbit Tyr204, pThr202 Polyclonal 0

General

Antigen Mitogen-Activated Protein Kinase 3 (MAPK3) Antikörper
Reaktivität Huhn, Rind (Kuh), Fruchtfliege (Drosophila melanogaster), Human, Maus, Ratte (Rattus), Schaf, Xenopus laevis
(549), (312), (288), (50), (40), (37), (37), (23), (19), (18), (18), (10), (10), (9), (9), (8), (7), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Wirt Kaninchen
(434), (119), (19), (6), (1)
Klonalität
Polyklonal
Konjugat Dieser MAPK3 Antikörper ist konjugiert mit Atto 633
(35), (26), (25), (15), (14), (14), (9), (9), (9), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2)
Applikation Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(439), (206), (199), (88), (75), (64), (56), (39), (37), (35), (15), (13), (8), (4), (3), (2), (1), (1), (1)
Pubmed 3 Publikationen vorhanden
Hersteller

Produktdetails anti-MAPK3 Antikörper

Target Details MAPK3 Anwendungsinformationen Handhabung Referenzen für anti-MAPK3 Antikörper (ABIN2486608) Bilder
Spezifität Detects ~44kda (ERK1) and ~42 kDa (ERK2).
Reinigung Peptide Affinity Purified
Immunogen A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
Plasmids, Primers & others

Target Details MAPK3

Produktdetails anti-MAPK3 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-MAPK3 Antikörper (ABIN2486608) Bilder zurück nach oben
Antigen
Andere Bezeichnung ERK1 (MAPK3 Antibody Abstract)
Hintergrund The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
Gen-ID 50689
NCBI Accession NP_059043
UniProt P21708
Pathways MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Anwendungsinformationen

Produktdetails anti-MAPK3 Antikörper Target Details MAPK3 Handhabung Referenzen für anti-MAPK3 Antikörper (ABIN2486608) Bilder zurück nach oben
Applikations-hinweise
  • WB (1:1000)
  • IHC (1:100)
  • ICC/IF (1:100)
  • FCM (1:100)
  • optimal dilutions for assays should be determined by the user.
Kommentare

A 1:1000 dilution of SPC-120 was sufficient for detection of ERK1/2 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MAPK3 Antikörper Target Details MAPK3 Anwendungsinformationen Referenzen für anti-MAPK3 Antikörper (ABIN2486608) Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C

Referenzen für anti-MAPK3 Antikörper (ABIN2486608)

Produktdetails anti-MAPK3 Antikörper Target Details MAPK3 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Yoon, Seger: "The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions." in: Growth factors (Chur, Switzerland), Vol. 24, Issue 1, pp. 21-44, 2006

Wolf: "Role of reactive oxygen species in angiotensin II-mediated renal growth, differentiation, and apoptosis." in: Antioxidants & redox signaling, Vol. 7, Issue 9-10, pp. 1337-45, 2005

Boulton, Gregory, Cobb: "Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase." in: Biochemistry, Vol. 30, Issue 1, pp. 278-86, 1991

Bilder

Produktdetails anti-MAPK3 Antikörper Target Details MAPK3 Anwendungsinformationen Handhabung Referenzen für anti-MAPK3 Antikörper (ABIN2486608) zurück nach oben
Bilder des Herstellers
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal A...
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Western blot analysis of Human Cell line lysates showing detection of ERK1 protein us...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: In...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ERK1 Polyclonal Ant...
Immunohistochemistry (IHC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody . Tissue: ba...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Mitogen-Activated Protein Kinase 3 (MAPK3) antibody (Atto 633) (ABIN2486608) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Erk1/2 Polyclonal A...