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ERK1 Antikörper (HRP)

MAPK3 Reaktivität: Ratte WB, IHC, IF, FACS, ICC Wirt: Kaninchen Polyclonal HRP
Produktnummer ABIN2486922
  • Target Alle ERK1 (MAPK3) Antikörper anzeigen
    ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
    Reaktivität
    • 255
    • 177
    • 147
    • 46
    • 20
    • 19
    • 18
    • 13
    • 12
    • 11
    • 11
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Ratte
    Wirt
    • 262
    • 29
    • 4
    • 1
    • 1
    Kaninchen
    Klonalität
    • 238
    • 59
    Polyklonal
    Konjugat
    • 122
    • 25
    • 22
    • 19
    • 13
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    Dieser ERK1 Antikörper ist konjugiert mit HRP
    Applikation
    • 249
    • 104
    • 86
    • 68
    • 65
    • 46
    • 42
    • 39
    • 36
    • 24
    • 18
    • 12
    • 12
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
    Spezifität
    Detects ~44kda (ERK1) and ~42 kDa (ERK2).
    Kreuzreaktivität
    Huhn, Rind (Kuh), Drosophila melanogaster, Human, Maus, Ratte, Schaf, Xenopus laevis
    Aufreinigung
    Peptide Affinity Purified
    Immunogen
    A 35 residue synthetic peptide, corresponding to Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
    Top Product
    Discover our top product MAPK3 Primärantikörper
  • Applikationshinweise
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • FCM (1:100)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    A 1:1000 dilution of ABIN2486922 was sufficient for detection of ERK1/2 in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Conjugated antibodies should be stored at 4°C
  • Target
    ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))
    Andere Bezeichnung
    ERK1 (MAPK3 Produkte)
    Synonyme
    ERK-1 antikoerper, ERK1 antikoerper, ERT2 antikoerper, HS44KDAP antikoerper, HUMKER1A antikoerper, P44ERK1 antikoerper, P44MAPK antikoerper, PRKM3 antikoerper, p44-ERK1 antikoerper, p44-MAPK antikoerper, Erk-1 antikoerper, Erk1 antikoerper, Ert2 antikoerper, Esrk1 antikoerper, Mnk1 antikoerper, Mtap2k antikoerper, Prkm3 antikoerper, p44 antikoerper, p44erk1 antikoerper, p44mapk antikoerper, ERK3 antikoerper, ERK6 antikoerper, P38GAMMA antikoerper, PRKM12 antikoerper, SAPK-3 antikoerper, SAPK3 antikoerper, fi06b09 antikoerper, wu:fi06b09 antikoerper, zERK1 antikoerper, Tb08.10J17.940 antikoerper, MAPK1 antikoerper, MNK1 antikoerper, AW123708 antikoerper, Erk6 antikoerper, P38gamma antikoerper, Prkm12 antikoerper, Sapk3 antikoerper, ATMAPK3 antikoerper, ATMPK3 antikoerper, T6D9.4 antikoerper, mitogen-activated protein kinase 3 antikoerper, mitogen-activated protein kinase 3 antikoerper, mitogen-activated protein kinase 12 antikoerper, mitogen activated protein kinase 3 antikoerper, mitogen-activated serine/threonine-protein kinase antikoerper, MAPK3 antikoerper, Mapk3 antikoerper, MAPK12 antikoerper, mapk3 antikoerper, Tc00.1047053509475.10 antikoerper, Tb927.8.3550 antikoerper, Mapk12 antikoerper, CEK1 antikoerper, MPK3 antikoerper
    Hintergrund
    The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5), however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).
    Gen-ID
    50689
    NCBI Accession
    NP_059043
    UniProt
    P21708
    Pathways
    MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteine
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