anti-Maus F2RL3 Antikörper für Western Blotting

Recommended F2RL3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3) Antikörper
  • F2RL3
  • PAR4
  • F2R like thrombin or trypsin receptor 3
  • F2R like thrombin/trypsin receptor 3
  • coagulation factor II (thrombin) receptor-like 3
  • F2RL3
  • F2rl3
Epitop
1st Extracellular Loop, AA 136-150, Cys149Ser-Mutant
16
15
11
9
6
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
123
60
58
3
2
2
1
1
1
1
1
1
Wirt
Kaninchen
110
15
1
Klonalität
Polyklonal
Konjugat
Dieser F2RL3 Antikörper ist unkonjugiert
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
70
42
33
26
26
9
7
6
2
1
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN1742017
$ 832.43
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.08196 ABIN223165 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.08196 ABIN1536071 IF ELISA WB Rabbit IgG AA 29-78 Anmelden zum Anzeigen Polyclonal 0
3.08196 ABIN3186327 ELISA IHC WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0
3.08196 ABIN3186328 ELISA IF WB Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal 0
3.08196 ABIN2736728 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.08196 ABIN5962728 ELISA IF WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.08196 ABIN5968382 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.08196 ABIN2801951 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 4
3.08196 ABIN3047159 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
3.08196 ABIN1853432 ELISA IF/ICC WB Rabbit Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN673224 IF (p) IHC (p) WB Rabbit IgG AA 320-343 Anmelden zum Anzeigen Polyclonal 1
1 ABIN2891147 IF ELISA WB Rabbit IgG AA 29-78 Anmelden zum Anzeigen Polyclonal 0
1 ABIN6288687 ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN1456758 ELISA WB Rabbit IgG AA 19-68 Anmelden zum Anzeigen Polyclonal 0
1 ABIN6268176 ELISA WB Rabbit IgG pThr163 Anmelden zum Anzeigen Polyclonal 0
1 ABIN6259627 ELISA ICC IF WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN673233 IHC (p) WB HRP Rabbit IgG AA 320-343 Anmelden zum Anzeigen Polyclonal 0
1 ABIN6268592 ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN673226 IHC (p) WB Biotin Rabbit IgG AA 320-343 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2892731 IF WB Rabbit Arg68 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3) Antikörper
Epitop 1st Extracellular Loop, AA 136-150, Cys149Ser-Mutant
(16), (15), (11), (9), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(123), (60), (58), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(110), (15), (1)
Klonalität
Polyklonal
Konjugat Dieser F2RL3 Antikörper ist unkonjugiert
(2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Applikation Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(70), (42), (33), (26), (26), (9), (7), (6), (2), (1), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-F2RL3 Antikörper

Target Details F2RL3 Anwendungsinformationen Handhabung Referenzen für anti-F2RL3 Antikörper (ABIN1742017) Bilder
Sequenz (C)HLRGQRWPFG EAA(S)R
Kreuzreaktivität (Details) Rat, mouse - identical.
Produktmerkmale Peptide Confirmation:
Confirmed by amino acid analysis.

Buffer after reconstitution:
Phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.025 % NaN3.

Antibody concentration after reconstitution:
0.8 mg/mL

Preadsorption Control:
1 µg peptide per 1 µg antibody.

GPCR
Reinigung Affinity purified on immobilized antigen.
Immunogen Peptide corresponding to amino acid residues 136-150 of human PAR-4. Cys 149 was replaced with Ser. 1st extracellular loop.
Plasmids, Primers & others

Target Details F2RL3

Produktdetails anti-F2RL3 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-F2RL3 Antikörper (ABIN1742017) Bilder zurück nach oben
Antigen
Andere Bezeichnung Protease-activated Receptor-4 (F2RL3 Antibody Abstract)
Hintergrund Synonyms:
PAR-4, Thrombin receptor like 3, Coagulation factor II receptor- like 3, F2RL3

Protease-activated receptor 4 (PAR-4) belongs to a family of four G protein-coupled receptors (PAR-1 - 4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.-3 Different PARs are activated by different proteases. Hence, PAR-4 is activated by both thrombin and trypsin whereas PAR-1 and PAR-3 are activated only by thrombin and PAR-2 is activated only by trypsis.-3 PAR-4 can be also cleaved and activated by other proteases such as cathepsin G. The intracellular signaling mechanisms mediated by PAR-4 activation are not completely elucidated but they involve calcium mobilization downstream of phospholipase Cbeta through the Galphaq pathwas.-3 Tissue distribution of PAR-4 is very broad with the highest expression levels found in lung, testis, pancreas and small intestine. In addition, PAR-4 expression was observed in platelets, megakaryocytes and leukocytes. Studies with platelets derived form PAR-4 knockout mice have established an essential role for PAR-4 in thrombin-induced platelet activation. PAR-4 is likely involved in other physiological functions such as regulation of gastrointestinal motility and regulation of vascular endothelial cell functios.-3
Gen-ID 9002
UniProt Q96RI0
Pathways Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process

Anwendungsinformationen

Produktdetails anti-F2RL3 Antikörper Target Details F2RL3 Handhabung Referenzen für anti-F2RL3 Antikörper (ABIN1742017) Bilder zurück nach oben
Applikations-hinweise Recommended Dilution:
WB: approx. 1:200,
IHC: approx. 1:100,
ICC: approx. 1:50,
FACS: approx. 1:40
Kommentare

Published Applications:
Western blot analysis (WB): - Rat DRGs (1:1000) (see Chen, D. et al. (2013)).
Immunohistochemistry (IH): - Rat DRG sections (1:200) (see Chen, D. et al. (2013)).
Immunocytochemistry (IC): - Rat dissociated DRGs (1:200) (see Chen, D. et al. (2013)).

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-F2RL3 Antikörper Target Details F2RL3 Anwendungsinformationen Referenzen für anti-F2RL3 Antikörper (ABIN1742017) Bilder zurück nach oben
Format Lyophilized
Rekonstitution 0.2 mL deionized water.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid multiple freezing and thawing.
Centrifuge all antibody preparations before use (10000 x g 5 min).
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The reconstituted solution can be stored at 4 °C for up to 2 weeks. For longer periods, small aliquots should be stored at -20 °C or below. The further dilutions should be made using a carrier protein such as BSA (1 %).

Referenzen für anti-F2RL3 Antikörper (ABIN1742017)

Produktdetails anti-F2RL3 Antikörper Target Details F2RL3 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Ossovskaya, Bunnett: "Protease-activated receptors: contribution to physiology and disease." in: Physiological reviews, Vol. 84, Issue 2, pp. 579-621, 2004

Bilder

Produktdetails anti-F2RL3 Antikörper Target Details F2RL3 Anwendungsinformationen Handhabung Referenzen für anti-F2RL3 Antikörper (ABIN1742017) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3) (1st Extracellular Loop), (AA 136-150), (Cys149Ser-Mutant) antibody (ABIN1742017) Western blot analysis of rat lung (lanes 1 and 3) and testis (lanes 2 and 4) lysates:...
Western Blotting (WB) image for anti-Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3) (1st Extracellular Loop), (AA 136-150), (Cys149Ser-Mutant) antibody (ABIN1742017) Western blot analysis of human prostate carcinoma PC3 (lanes 1 and 4), and LNCaP (lan...
Immunohistochemistry (IHC) image for anti-Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3) (1st Extracellular Loop), (AA 136-150), (Cys149Ser-Mutant) antibody (ABIN1742017) Expression of PAR-4 in rat lung Immunohistochemical staining of rat lung paraffin-emb...
Immunohistochemistry (IHC) image for anti-Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3) (1st Extracellular Loop), (AA 136-150), (Cys149Ser-Mutant) antibody (ABIN1742017) Expression of PAR-4 in rat epididymis Immunohistochemical staining of rat epididymis ...
Immunocytochemistry (ICC) image for anti-Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3) (1st Extracellular Loop), (AA 136-150), (Cys149Ser-Mutant) antibody (ABIN1742017) Expression of PAR-4 in a human breast adenocarcinoma cell line Immunocytochemical sta...
Flow Cytometry (FACS) image for anti-Coagulation Factor II (Thrombin) Receptor-Like 3 (F2RL3) (1st Extracellular Loop), (AA 136-150), (Cys149Ser-Mutant) antibody (ABIN1742017) Anti-Protease-Activated_Receptor-4_(extracellular) - Indirect Flow cytometry analysis...