GFP Selector

Details zu Produkt Nr. ABIN5311507, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Reaktivität
Aequorea victoria
Wirt
Camelid (Camelidae)
Applikation
Protein Complex Immunoprecipitation (Co-IP), Immunoprecipitation (IP), Purification (Purif), Chromatin Immunoprecipitation (ChIP), RNA-Binding Protein Immunoprecipitation (RIP)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Proben Cell Extracts
Spezifität The Antibody with Clone 1H1 recognizes GFP, mEGFP, superfolder GFP, Emerald GFP and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.
Produktmerkmale GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4 % cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GFP Selector thus features high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background. GFP Selector immobilizes a wide range of GFP derivatives. For details see our Product Specificity Chart below. GFP Selector is compatible not only with physiological buffers but also with high stringency buffers. GFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
Bestandteile 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
Benötigtes Material wash buffers, columns, tubes
Bead Ligand Antibody
Bead Matrix Agarose beads
Bead Size 90 μm
Hintergrund Green fluorescent protein GFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse GFP to the N- or the C-terminus of the p
Applikations-hinweise 200 μL slurry for up to 10 reactions
Kommentare

Capacity: > 4 μg GFP per μl of packed beads (i.e. 2 μl of slurry)

Beschränkungen Nur für Forschungszwecke einsetzbar
Buffer 50 % slurry in 20 % ethanol / PBS 0.09 % sodium azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Bilder des Herstellers
Immunoprecipitation (IP) image for GFP Selector (ABIN5311507) A) Pull-down of GFP from a mixture of GFP, mCherry and mTagBFP B) Immunoprecipitation...