GFP-Catcher

Produktnummer ABIN5311508

Produktdetails

Highlights:

• High-affinity single-domain antibody
• Recognizes GFP and many GFP derivatives
• Bead size 50-150 μm

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Aequorea victoria

Wirt: Camelid (Camelidae)

Applikation: Protein Complex Immunoprecipitation (Co-IP), Immunoprecipitation (IP), Purification (Purif), Chromatin Immunoprecipitation (ChIP), RNA-Binding Protein Immunoprecipitation (RIP)

Verwendungszweck: GFP-Catcher is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads.

Proben: Cell Extracts

Spezifität: Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine and most common CFP and YFP variants.

Kreuzreaktivität (Details): Does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives.

Produktmerkmale: GFP-Catcher is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4 % cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer.
GFP-Catcher thus features high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background.
GFP-Catcher immobilizes a wide range of GFP derivatives.
GFP-Catcher is compatible not only with physiological buffers but also with high stringency buffers.
GFP-Catcher thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

Bestandteile: 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody

Benötigtes Material: wash buffers, columns, tubes

Bead Ligand: Antibody

Bead Matrix: Agarose beads

Bead Size: 90 μm

Anwendungsinformationen

Applikationshinweise: Coating: sdAb anti-GFP clone 1H1
Matrix: 4 % cross-linked agarose, bead size 50-150 μm
Capacity: > 4 μg GFP per μl of packed beads (= 2 μL of slurry)
Buffer Compatibility:

• Common buffer substances at pH 5 to 9
• 2 % Triton X-100, 1 % Tween-20, 1 % NP-40, 1 % CHAPS, 1 % Deoxycholate, 0.1 % SDS
• 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA
• 4 M urea
• 10 mM DTT, 10 mM 2-Mercaptoethanol
• RNAse A, DNAse I, Benzonase, protease inhibitors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 50 % slurry in PBS containing 20 % Ethanol

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4 °C, do not freeze

Haltbarkeit: 12 months

Antigendetails

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP (GFP Produkte)

Synonyme: green fluorescent protein Bead, gfp Bead

Substanzklasse: Viral Protein