anti-Ratte (Rattus) FASL Antikörper für Flow Cytometry

Recommended FASL Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antikörper
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • FASL
  • TNFSF6
  • fasl
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • FASLG
  • zgc:162027
  • Fas ligand
  • Fas ligand L homeolog
  • Fas ligand (TNF superfamily, member 6)
  • FASLG
  • faslg.L
  • Fasl
  • Faslg
  • faslg
Reaktivität
Maus, Ratte (Rattus)
438
174
85
36
7
5
5
3
3
2
1
Wirt
Armenischer Hamster
278
171
49
33
31
9
1
Klonalität
Monoklonal
Konjugat
Dieser FASL Antikörper ist unkonjugiert
47
32
29
14
13
13
10
10
9
9
9
9
9
9
8
7
7
3
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Blocking Reagent (BR), Flow Cytometry (FACS)
337
261
127
100
76
70
52
48
29
28
13
12
10
10
9
5
2
2
2
1
1
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN1176980
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
14.007843 ABIN614573 FACS FITC Hamster IgG Anmelden zum Anzeigen MFL4 3
14.007843 ABIN614579 FACS PE Hamster IgG Anmelden zum Anzeigen MFL4 3
14.007843 ABIN2478831 FACS Hamster IgG Anmelden zum Anzeigen MFL4 3
14.007843 ABIN2665564 FACS IF IHC Hamster IgG Anmelden zum Anzeigen MFL4 6
14.007843 ABIN447421 FACS FITC Armenian Hamster IgG Anmelden zum Anzeigen MFL4 0
14.007843 ABIN2660964 FACS Biotin Hamster IgG Anmelden zum Anzeigen MFL4 6
1 ABIN2689023 BR FACS Armenian Hamster IgG3, kappa Anmelden zum Anzeigen MFL4 11
1 ABIN2689026 FACS Biotin Armenian Hamster IgG3, kappa Anmelden zum Anzeigen MFL4 10
1 ABIN614575 Func FACS Hamster IgG Anmelden zum Anzeigen MFL4 3
1 ABIN614577 FACS Hamster IgG Anmelden zum Anzeigen MFL4 3
1 ABIN726420 FACS PE Rabbit IgG AA 170-220 Anmelden zum Anzeigen Polyclonal 0
1 ABIN1820435 FACS Biotin Hamster IgG Anmelden zum Anzeigen MFL4 0
1 ABIN2478838 Func FACS Hamster IgG Anmelden zum Anzeigen MFL4 3
1 ABIN1819963 FACS PE Hamster IgG Anmelden zum Anzeigen MFL4 0
1 ABIN1819962 FACS Hamster IgG Anmelden zum Anzeigen MFL4 0
1 ABIN1820310 FACS Hamster IgG Anmelden zum Anzeigen 0
1 ABIN1820315 FACS Hamster IgG Anmelden zum Anzeigen 0
1 ABIN2478836 FACS FITC Hamster IgG Anmelden zum Anzeigen MFL4 3
1 ABIN2478834 FACS Alexa Fluor 647 Hamster IgG Anmelden zum Anzeigen MFL4 3
1 ABIN2478839 FACS PE Hamster IgG Anmelden zum Anzeigen MFL4 3

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antikörper
Reaktivität Maus, Ratte (Rattus)
(438), (174), (85), (36), (7), (5), (5), (3), (3), (2), (1)
Wirt Armenischer Hamster
(278), (171), (49), (33), (31), (9), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser FASL Antikörper ist unkonjugiert
(47), (32), (29), (14), (13), (13), (10), (10), (9), (9), (9), (9), (9), (9), (8), (7), (7), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Applikation Blocking Reagent (BR), Flow Cytometry (FACS)
(337), (261), (127), (100), (76), (70), (52), (48), (29), (28), (13), (12), (10), (10), (9), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 11 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FASL Antikörper

Target Details FASL Anwendungsinformationen Handhabung Referenzen für anti-FASL Antikörper (ABIN1176980) Bilder
Marke BD Pharmingen™
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterilität 0.2 μm filtered
Endotoxin-Niveau Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Mouse FasL transfected cells
Klon MFL4
Isotyp IgG3, kappa

Target Details FASL

Produktdetails anti-FASL Antikörper Anwendungsinformationen Handhabung Referenzen für anti-FASL Antikörper (ABIN1176980) Bilder zurück nach oben
Antigen
Andere Bezeichnung CD178 (FASL Antibody Abstract)
Hintergrund The MFL4 antibody, generated against mouse CD178 (Fas Ligand, CD95 Ligand), cross-reacts with the rat Fas Ligand antigen. MFL4 mAb detects rat Fas Ligand expressed by transfected cells, but not the parental cell line. In addition, the epitope detected by MFL4 can be induced on activated rat splenic T lymphocytes. The MFL4 mAb has been reported to inhibit killing of mouse lymphoma line A20.2J by rat FasL-transfected cells. The tissue distribution of mouse Fas Ligand has been well characterized. It is expressed at the mRNA and protein levels in a variety of tissues and cell types, including spleen, bone marrow, testis, uterus, eye, and activated lymphocytes. The expression of Fas Ligand in the rat has been detected in liver Kupffer cells, T cells in the central nervous system, spleen, and corpus luteum. MFL4 antibody reacts with the mouse Fas Ligand protein in a pattern similar to that of MFL3 antibody (Cat. No. 555291). Fas Ligand is a type-II transmembrane protein, a member of the TNF/NGF superfamily and a ligand for Fas (CD95). Fas Ligand and its counter-receptor CD95 are believed to participate in T-cell development, the regulation of immune responses, and cell-mediated cytotoxic mechanisms. It has been reported that human Fas Ligand is released from the surface of activated lymphocytes by metalloproteinase.
Synonyms: Fas Ligand, CD95 Ligand
Pathways Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Anwendungsinformationen

Produktdetails anti-FASL Antikörper Target Details FASL Handhabung Referenzen für anti-FASL Antikörper (ABIN1176980) Bilder zurück nach oben
Applikationshinweise We have found that enriched splenic T cells are induced to express Fas Ligand by 6 - 8-hour culture with plate-bound anti-alphabeta TCR mAb R73 (Cat. No. 554910) and anti-rat CD28 mAb JJ316 (Cat. No. 554992). Because Fas Ligand is expressed at low density on activated cells, we recommend the use of a biotinylated second-step antibody, such as polyclonal goat anti-hamster IgG, with a bright third-step reagent, such as Streptavidin-PE (Cat. No. 554061). Other reported applications include blocking of the cytotoxic activities of mFasL-transfected L5178Y T lymphoma, rFasL-transfected COS cells, and rFasL-transfected L5178Y cells.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Referenzen für anti-FASL Antikörper (ABIN1176980) Bilder zurück nach oben
Konzentration 1.0 mg/mL
Buffer Aqueous buffered solution containing no preservative, 0.2μm filtered. Endotoxin level is ≤0.01 ng/μg of protein.
Konservierungsmittel Azide free
Lagerung 4 °C
Informationen zur Lagerung This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store undiluted at 4°C.

Referenzen für anti-FASL Antikörper (ABIN1176980)

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Trinité, Voisine, Yagita, Josien: "A subset of cytolytic dendritic cells in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 8, pp. 4202-8, 2000

Moalem, Monsonego, Shani, Cohen, Schwartz: "Differential T cell response in central and peripheral nerve injury: connection with immune privilege." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 13, Issue 10, pp. 1207-17, 1999

Müschen, Warskulat, Douillard, Gilbert, Häussinger: "Regulation of CD95 (APO-1/Fas) receptor and ligand expression by lipopolysaccharide and dexamethasone in parenchymal and nonparenchymal rat liver cells." in: Hepatology (Baltimore, Md.), Vol. 27, Issue 1, pp. 200-8, 1998

White, McCombe, Pender: "The roles of Fas, Fas ligand and Bcl-2 in T cell apoptosis in the central nervous system in experimental autoimmune encephalomyelitis." in: Journal of neuroimmunology, Vol. 82, Issue 1, pp. 47-55, 1998

Kayagaki, Yamaguchi, Nagao, Matsuo, Maeda, Okumura, Yagita: "Polymorphism of murine Fas ligand that affects the biological activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3914-9, 1997

Griffith, Brunner, Fletcher, Green, Ferguson: "Fas ligand-induced apoptosis as a mechanism of immune privilege." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1189-92, 1996

Kayagaki, Kawasaki, Ebata, Ohmoto, Ikeda, Inoue, Yoshino, Okumura, Yagita: "Metalloproteinase-mediated release of human Fas ligand." in: The Journal of experimental medicine, Vol. 182, Issue 6, pp. 1777-83, 1996

Suda, Okazaki, Naito, Yokota, Arai, Ozaki, Nakao, Nagata: "Expression of the Fas ligand in cells of T cell lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3806-13, 1995

Brunner, Mogil, LaFace, Yoo, Mahboubi, Echeverri, Martin, Force, Lynch, Ware: "Cell-autonomous Fas (CD95)/Fas-ligand interaction mediates activation-induced apoptosis in T-cell hybridomas." in: Nature, Vol. 373, Issue 6513, pp. 441-4, 1995

Vignaux, Vivier, Malissen, Depraetere, Nagata, Golstein: "TCR/CD3 coupling to Fas-based cytotoxicity." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 781-6, 1995

Smith, Farrah, Goodwin: "The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death." in: Cell, Vol. 76, Issue 6, pp. 959-62, 1994

Bilder

Produktdetails anti-FASL Antikörper Target Details FASL Anwendungsinformationen Handhabung Referenzen für anti-FASL Antikörper (ABIN1176980) zurück nach oben
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (ABIN1176980) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody