TOMM20 Antikörper (AA 47-145)

Produktnummer ABIN968798

Produktdetails anti-TOMM20 Antikörper

Target: TOMM20 (Mitochondrial Import Receptor Subunit TOM20 homolog (TOMM20))

Bindungsspezifität: AA 47-145

Reaktivität: Human, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TOMM20 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Hund, Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Tom20 aa. 47-145

Klon: 29-Tom20

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968537, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-TOMM20 Antikörper (ABIN968798)

Krimmer, Rapaport, Ryan, Meisinger, Kassenbrock, Blachly-Dyson, Forte, Douglas, Neupert, Nargang, Pfanner: "Biogenesis of porin of the outer mitochondrial membrane involves an import pathway via receptors and the general import pore of the TOM complex." in: The Journal of cell biology, Vol. 152, Issue 2, pp. 289-300, (2001) (PubMed).

Abe, Shodai, Muto, Mihara, Torii, Nishikawa, Endo, Kohda: "Structural basis of presequence recognition by the mitochondrial protein import receptor Tom20." in: Cell, Vol. 100, Issue 5, pp. 551-60, (2000) (PubMed).

Hernández, Giner, Hernández-Yago: "Gene structure of the human mitochondrial outer membrane receptor Tom20 and evolutionary study of its family of processed pseudogenes." in: Gene, Vol. 239, Issue 2, pp. 283-91, (1999) (PubMed).

Details zu TOMM20

Target: TOMM20 (Mitochondrial Import Receptor Subunit TOM20 homolog (TOMM20))

Andere Bezeichnung: Tom20 (TOMM20 Produkte)

Synonyme: MAS20 antikoerper, MOM19 antikoerper, TOM20 antikoerper, 1810060K07Rik antikoerper, BB284719 antikoerper, Gm19268 antikoerper, mKIAA0016 antikoerper, tomm20 antikoerper, wu:fk47b11 antikoerper, zgc:73165 antikoerper, mas20 antikoerper, mom19 antikoerper, tom20 antikoerper, zgc:92628 antikoerper, translocase of outer mitochondrial membrane 20 antikoerper, translocase of outer mitochondrial membrane 20 homolog (yeast) antikoerper, Mitochondrial import receptor subunit TOM20 homolog antikoerper, translocase of outer mitochondrial membrane 20 homolog L homeolog antikoerper, translocase of outer mitochondrial membrane 20b antikoerper, TOMM20 antikoerper, Tomm20 antikoerper, tomm20a antikoerper, tomm-20 antikoerper, tomm20.L antikoerper, tomm20b antikoerper

Hintergrund: Many mitochondrial proteins are encoded by nuclear genes and synthesized in the cytosol as pre-proteins. These pre-proteins are targeted to the mitochondria where they are translocated across the outer and inner membranes of the mitochondria. At the outer membrane of the mitochondria, this process requires a multiprotein complex called the Translocase of the Outer Membrane of the mitochondria (TOM). The TOM complex includes receptor components, Tom20, 22, 37, and 70, as well as a multisubunit general import pore complex of 400 kDa. Tom20 contains a hydrophobic N-terminal region that anchors it to the outer membrane of the mitochondria, and a cytosolic region that contains a domain that facilitates binding to the basic amphiphilic targeting sequences of pre-proteins and a tetratricopeptide repeat (TPR) motif involved in interactions with Tom70. In yeast, Tom20 and Tom22 mediate the import of all pre-proteins known to use the general import machinery of the mitochondria. Thus, Tom20 is an important receptor for many mitochondrial pre-proteins that use the outer mitochondrial translocation complex.

Molekulargewicht: 18 kDa

Pathways: Unfolded Protein Response