Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (Biotin) - Preadsorbed
-
- Target Alle IgG Produkte
- IgG
- Fragment
- F(ab')2 fragment
-
Bindungsspezifität
- Heavy & Light Chain
-
Reaktivität
- Maus
-
Wirt
- Ziege
-
Klonalität
- Polyklonal
-
Konjugat
- Biotin
-
Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgG and Mouse Serum.
- Aufreinigung
- Preadsorption: Solid phase absorption
- Sterilität
- Sterile filtered
- Immunogen
-
Immunogen: Mouse IgG whole molecule
- Isotyp
- IgG
-
-
- Applikationshinweise
-
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
ELISA Dilution: 1:15,000 - 1:60,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
-
Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid cycles of freezing and thawing.
- Lagerung
- RT,4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
- Haltbarkeit
- 12 months
-
- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
-
Synonyms: Goat F(ab')2 Anti-Mouse IgG Antibody Biotin Conjugation, Goat Fab2 Anti-Mouse IgG Biotin Conjugated Antibody
Background: F(ab')2 Anti-Mouse IgG (H&L) Biotin Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
-