Mitotic Cells Antikörper

Produktnummer ABIN959451

Produktdetails

Target: Mitotic Cells

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Spezifität: Strongly stains mitotic cells.

Aufreinigung: Purified

Immunogen: Total cell lysate of the human bladder carcinoma cell line T24

Klon: 8B3G

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:50 - 1:100), ICC (1:50 - 1:100), IHC

Usage: Strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) antibody clearly distinguishes these M-phase cells from cell at other stages of the cell cycle. Dynamic information can be obtained by combining BrdU incorporation with antibody staining, which can distinguish and quantitate the four major fractions of the cell cycle.

Not recommended for: IHC-P, WB

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS containing 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: avoid freeze thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Antigendetails

Target: Mitotic Cells