Mitotic Cells Antikörper

Produktnummer ABIN112395

Produktdetails

Target: Mitotic Cells

Reaktivität: Human, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Kreuzreaktivität (Details): Species reactivity (tested):Human, Zebrafish.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Total cell lysate of the human bladder carcinoma cell line T24.

Klon: 8B3G

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: This antibody is suitable for Flow cytometric analyses (1/50-1/100) andImmunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent (1/50-1/100). Not suitable for Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the lyophilized antibody at 2-8 °C for up to one month or at -20 °C for longer.

Antigendetails

Target: Mitotic Cells

Hintergrund: The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques, such as the BrdU incorporation assay. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a 'quiescent' phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S, and from G2 to M-phase. M phase itself is highly regulated, and is divided into five stages: prophase, prometaphase, metaphase, telophase and anaphase.