TUBB3 Antikörper (Tubulin, Beta, 3) (N-Term) (FITC)

Details for Product anti-TUBB3 Antibody No. ABIN93912, Anbieter: Anmelden zum Anzeigen
Antigen
  • CDCBM
  • CFEOM3A
  • TUBB4
  • beta-4
  • TUBB3
  • 3200002H15Rik
  • M(beta)3
  • M(beta)6
  • tubb3
  • tubulin beta 3 class III
  • tubulin, beta 3 class III
  • tubulin beta 3 class III L homeolog
  • beta-tubulin 3
  • tubulin beta-3 chain
  • beta tubulin 3
  • TUBB3
  • Tubb3
  • tubb3
  • tubb3.L
  • LOC100581245
  • tub3
Epitop
N-Term, C-Term, AA 441-448
49
42
23
16
15
8
6
5
4
4
4
4
3
3
2
2
1
1
1
1
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Reaktivität
Alle Spezies
272
134
116
20
19
15
14
9
8
7
7
6
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4
4
3
2
2
2
2
1
1
1
1
1
1
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1
Wirt
Maus
150
137
19
16
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser TUBB3 Antikörper ist konjugiert mit FITC
23
14
12
5
5
5
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2
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2
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2
2
1
1
1
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1
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1
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1
Applikation
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
280
145
93
71
69
60
55
21
17
13
13
11
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2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Klon TU-20
Isotyp IgG1
Spezifität The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
Produktmerkmale The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Plasmids, Primers & others Plasmide, Primers & weitere TUBB3 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung TUBB3 (TUBB3 Antibody Abstract)
Hintergrund The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Pathways Microtubule Dynamics, M Phase
Applikations-hinweise Immunocytochemistry on fixed and permeabilized cells. Suggested working dilution is 1:40.The conjugate was also successfully used on paraffin sections using confocal microscopy. It is recommended that the user titrates the reagent for use in the particular testing system.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Avoid prolonged exposure to light.
Lagerung 4 °C
Informationen zur Lagerung Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Tubulin, Beta, 3 (TUBB3) (AA 441-448), (C-Term), (N-Term) antibody (FITC) (ABIN93912) Immunofluorescence staining (mouse neuroblastoma cells) Immunofluorescence staining o...
Immunofluorescence (IF) image for anti-Tubulin, Beta, 3 (TUBB3) (AA 441-448), (C-Term), (N-Term) antibody (FITC) (ABIN93912) Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to...
Immunofluorescence (IF) image for anti-Tubulin, Beta, 3 (TUBB3) (AA 441-448), (C-Term), (N-Term) antibody (FITC) (ABIN93912) Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence stai...
Produkt verwendet in: Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012 (PubMed).

Jirásek, Cipro, Musilová, Kubecová, Mandys: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, 2009 (PubMed).

Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, 2009 (PubMed).

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008 (PubMed).

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007 (PubMed).

Jirásek, Písaríková, Viklický, Mandys: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, 2007 (PubMed).

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed).

P?knicová, Kubátová, Sulimenko, Dráberová, Viklický, Hozák, Dráber: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001 (PubMed).

Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000 (PubMed).

Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed).

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