Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (AA 101-200) Antikörper (Alexa Fluor 488)

Produktnummer ABIN913846

Produktdetails

Target: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Bindungsspezifität: AA 101-200

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Alexa Fluor 488

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RNF56/CBL-B

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Andere Bezeichnung: RNF56 (CBLB Produkte)

Synonyme: rnf56 antikoerper, cblb antikoerper, AI429560 antikoerper, AI851073 antikoerper, Cbl-b antikoerper, Cbl proto-oncogene B, E3 ubiquitin protein ligase S homeolog antikoerper, Cbl proto-oncogene B, E3 ubiquitin protein ligase L homeolog antikoerper, Casitas B-lineage lymphoma b antikoerper, Cbl proto-oncogene B antikoerper, cblb.S antikoerper, cblb.L antikoerper, Cblb antikoerper, CBLB antikoerper

Hintergrund:

Synonyms: Cbl-b, RNF56, Nbla00127, E3 ubiquitin-protein ligase CBL-B, Casitas B-lineage lymphoma proto-oncogene b, RING finger protein 56, SH3-binding protein CBL-B, Signal transduction protein CBL-B, CBLB

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. Slightly promotes SRC ubiquitination. May be involved in EGFR ubiquitination and internalization. May be functionally coupled with the E2 ubiquitin-protein ligase UB2D3.

Gen-ID: 868

UniProt: Q13191