LATS2 Antikörper (pSer83)

Produktnummer ABIN753568

Produktdetails anti-LATS2 Antikörper

Target: LATS2 (LATS, Large Tumor Suppressor, Homolog 2 (Drosophila) (LATS2))

Bindungsspezifität: pSer83

Reaktivität: Maus, Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LATS2 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This phosphorylation site is homologous to that of Ser82 in Mouse and Ser83 Rat.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Dog,Cow,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human LATS2 around the phosphorylation site of Ser83

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-LATS2 Antikörper (ABIN753568)

Zhang, Wang, Xie, Chen, Sinkemani, Yu, Wang, Mao, Wu: "Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence." in: Oncotarget, Vol. 9, Issue 2, pp. 2175-2192, (2018) (PubMed).

Details zu LATS2

Target: LATS2 (LATS, Large Tumor Suppressor, Homolog 2 (Drosophila) (LATS2))

Andere Bezeichnung: LATS2 (LATS2 Produkte)

Synonyme: 4932411G09Rik antikoerper, AV277261 antikoerper, AW228608 antikoerper, KPM antikoerper, lats2 antikoerper, large tumor suppressor kinase 2 antikoerper, large tumor suppressor 2 antikoerper, LATS2 antikoerper, Lats2 antikoerper, lats2 antikoerper

Hintergrund:

Synonyms: KPM, Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Warts-like kinase, LATS2

Background: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.

Gen-ID: 26524

UniProt: Q9NRM7