MEK1 Antikörper (C-Term) (Biotin)

Produktnummer ABIN7565793

Der Biotin-konjugierte Maus Monoklonal anti-MEK1 Antikörper (Klon 13B6-G12) (ABIN7565793) detektiert spezifisch MEK1 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für MEK1 Antikörper (C-Term) (Biotin) (ABIN7565793)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MEK1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Klon: 13B6-G12

Produktdetails anti-MEK1 Antikörper (Biotin)

Bindungsspezifität: C-Term

Verwendungszweck: MEK1 C-Term Antibody Biotin

Kreuzreaktivität (Details): This biotin conjugated protein A purified mouse monoclonal antibody reacts specifically with human MEK1.

Produktmerkmale: Synonyms: mouse anti-MEK1 antibody biotin conjugation, biotin conjugated mouse anti-MEK1 antibody, MAP2K, MEK, MEK 1, MKK1, PRKMK1, MEK-1 Antibody

Aufreinigung: Anti-MEK1 is purified from tissue culture supernatant by protein A purification.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-MEK1 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotyp: IgG1 kappa

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-MEK 1 Biotin Conjugated (MOUSE) Antibody has been tested by Western Blotting and ELISA.  Specific conditions of reactivity should be optimized by the end user.  Expect a band of approximately 43.5 kDa. Western Blot Dilution: 1 μg/mL ELISA Dilution: 1:40,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Details zu MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Andere Bezeichnung: MAP2K1

Hintergrund: Background: MEK1 antibodies detect the MEK1 isoform.  Mitogen-activated protein kinase kinase 1, also known as MKK or MEK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain.  Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase.   MEK1 and MEK2 are about 80 % identical to each other, and nearly identical within the kinase domain.   The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: Q02750

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling