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MEK1 Antikörper (AA 2-150)

MAP2K1 Reaktivität: Human ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN686482
  • Target Alle MEK1 (MAP2K1) Antikörper anzeigen
    MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
    Bindungsspezifität
    • 46
    • 28
    • 28
    • 23
    • 22
    • 22
    • 20
    • 19
    • 18
    • 15
    • 15
    • 14
    • 12
    • 11
    • 10
    • 8
    • 7
    • 7
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-150
    Reaktivität
    • 375
    • 206
    • 200
    • 37
    • 30
    • 18
    • 16
    • 12
    • 12
    • 10
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 339
    • 33
    • 3
    • 2
    • 2
    Kaninchen
    Klonalität
    • 292
    • 87
    Polyklonal
    Konjugat
    • 180
    • 25
    • 22
    • 17
    • 15
    • 14
    • 14
    • 12
    • 12
    • 12
    • 12
    • 10
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MEK1 Antikörper ist unkonjugiert
    Applikation
    • 247
    • 123
    • 103
    • 67
    • 65
    • 46
    • 32
    • 32
    • 25
    • 14
    • 11
    • 10
    • 9
    • 7
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Kreuzreaktivität
    Human
    Homologie
    Mouse,Rat,Dog,Pig,Chicken,Rabbit
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human MEK1
    Isotyp
    IgG
  • Applikationshinweise
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #029646 (Immunohistochemistry)
    'Independent Validation' Siegel
    by
    Immunohistochemistry Core, NYU Langone
    No.
    #029646
    Datum
    28.03.2014
    Antigen
    Chargennummer
    90915
    Validierte Anwendung
    Immunohistochemistry
    Positivkontrolle
    Human placenta
    Negativkontrolle
    Human breast adipocytes
    Bewertung
    Signal detected in positive control sample and not in negative control sample.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    • Antigen: Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1)
    • Catalog number: ABIN686482
    • Lot number: 90915
    Sekundärantikörper
    • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
    • Lot number: D07640BA
    Full Protocol
    • Immunohistochemistry was performed on a Ventana NEXes automated platform; instrument manufacturer specific reagents are italicized.
    • 1. Slides were preheated in convection oven at 60°C for 30 min
    • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
    • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
    • 4. NEXes instrument procedure, iView DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
    • 5. Slides rinsed with *reaction buffer* x3
    • 6. *iView Inhibitor (H2O2)* applied and incubated for 4 min
    • 7. Slides rinsed with *reaction buffer*
    • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 microliter applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
    • 9. Slides rinsed with *reaction buffer* x3
    • 10. *iView Biotinylated IgG* applied and incubated for 8 min
    • 11. Slides rinsed with *reaction buffer*
    • 14. *iView Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
    • 15. Slides rinsed with *reaction buffer*
    • 16. *iView DAB/H2O2* applied and incubated for 8 min
    • 17. Slides rinsed with *reaction buffer*
    • 18. *iView Copper* applied and incubated for 4 min
    • 19. Slides rinsed with *reaction buffer*
    • 20. Slides washed in Dawn Detergent/tap water
    • 21. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 sec - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
    • 22. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
    • 23. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
    Anmerkungen
    • Deviations from protocol/procedure supplied by manufacturer:
    • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
    • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
    • Step 3.1: Slides were heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
    • Step 3.2: Slides were cooled for 30 minutes; manufacturer cools for 20 minutes.
    • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
    • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
    • Step 8.1: Antibody diluted in PBS at 1:250; manufacture did not recommend diluent or dilution.
    • Step 8.2.1: Primary antibody incubated at room temperature overnight; manufacturer incubates overnight 4°C with agitation.
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Haltbarkeit
    12 months
  • Target
    MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
    Andere Bezeichnung
    MEK1 (MAP2K1 Produkte)
    Synonyme
    Mek1 antikoerper, CFC3 antikoerper, MAPKK1 antikoerper, MEK1 antikoerper, MKK1 antikoerper, PRKMK1 antikoerper, MEKK1 antikoerper, Prkmk1 antikoerper, mek-2 antikoerper, si:ch211-242m18.2 antikoerper, wu:fj56a12 antikoerper, wu:fj61b01 antikoerper, zgc:56557 antikoerper, ATMEK1 antikoerper, F20B18.180 antikoerper, F20B18_180 antikoerper, MAP kinase/ ERK kinase 1 antikoerper, MITOGEN ACTIVATED PROTEIN KINASE KINASE antikoerper, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 antikoerper, NMAPKK antikoerper, MEK antikoerper, mitogen activated protein kinase kinase 1 antikoerper, mitogen-activated protein kinase kinase 1 antikoerper, mitogen-activated protein kinase kinase 1 L homeolog antikoerper, MAP kinase/ ERK kinase 1 antikoerper, Map2k1 antikoerper, MAP2K1 antikoerper, map2k1.L antikoerper, map2k1 antikoerper, MEK1 antikoerper
    Hintergrund

    Synonyms: CFC3, MEK1, MKK1, MAPKK1, PRKMK1, Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1

    Background: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

    Gen-ID
    5604
    UniProt
    Q02750
    Pathways
    MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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