PRKDC Antikörper

Produktnummer ABIN7181005

Produktdetails anti-PRKDC Antikörper

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKDC Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Kreuzreaktivität: Human, Maus

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from Human DNA-PK.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Andere Bezeichnung: PRKDC (PRKDC Produkte)

Synonyme: DNA-PKcs antikoerper, DNAPK antikoerper, DNPK1 antikoerper, HYRC antikoerper, HYRC1 antikoerper, XRCC7 antikoerper, p350 antikoerper, DNA-PKCS antikoerper, dna-pkcs antikoerper, dnapk antikoerper, dnpk1 antikoerper, hyrc antikoerper, hyrc1 antikoerper, prkdc-A antikoerper, xrcc7 antikoerper, PRKDC antikoerper, AI326420 antikoerper, AU019811 antikoerper, DNAPDcs antikoerper, DOXNPH antikoerper, dxnph antikoerper, p460 antikoerper, scid antikoerper, slip antikoerper, protein kinase, DNA-activated, catalytic polypeptide antikoerper, protein kinase, DNA-activated, catalytic polypeptide L homeolog antikoerper, DNA-dependent protein kinase catalytic subunit antikoerper, protein kinase, DNA activated, catalytic polypeptide antikoerper, PRKDC antikoerper, prkdc.L antikoerper, LOC100633457 antikoerper, Prkdc antikoerper

Hintergrund:

Background: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2, negatively regulates CRY1 phosphorylation.

Wesley D. Block, Nucleic Acids Res., Apr 2004, 32: 1967 - 1972.
Katherine S. Pawelczak, Nucleic Acids Res., Jan 2005, 33: 152 - 161.
Eun-Jung Park, Nucleic Acids Res., Dec 2003, 31: 6819 - 6827.
Suisheng Zhang, Nucleic Acids Res., Jan 2004, 32: 1 - 10.

Aliases: DNA dependent protein kinase catalytic subunit antibody, DNA PK catalytic subunit antibody, DNA-dependent protein kinase catalytic subunit antibody, DNA-PK catalytic subunit antibody, DNA-PKcs antibody, DNAPK antibody, DNAPK catalytic subunit antibody, DNPK 1 antibody, DNPK1 antibody, Hyper radiosensitivity of murine scid mutation, complementing 1 antibody, Hyperradiosensitivity complementing 1, mouse, homolog of 1 antibody, HYRC 1 antibody, HYRC antibody, HYRC1 antibody, IMD26 antibody, p350 antibody, p460 antibody, PKRDC antibody, PRKDC antibody, PRKDC_HUMAN antibody, Protein Kinase DNA Activated Catalytic Polypeptide antibody, XRCC 7 antibody, XRCC7 antibody

UniProt: P78527

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response