SMARCB1 Antikörper

Produktnummer ABIN6972743

Produktdetails anti-SMARCB1 Antikörper

Target: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SMARCB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktmerkmale: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily B, Member 1) is a core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. SMARCB1 antibody (mAb) (Clone 2C2) was raised in a Mouse host. It has been validated for use in Immunocytochemistry, Immunofluorescence and Western blot, it has been shown to react with Human samples.

Aufreinigung: Protein A Chromatography

Immunogen: This antibody was raised against full-length recombinant human SMARCB1 protein.

Klon: 2C2

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Details zu SMARCB1

Target: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Andere Bezeichnung: SMARCB1 (SMARCB1 Produkte)

Synonyme: SMARCB1 antikoerper, BAF47 antikoerper, INI1 antikoerper, MRD15 antikoerper, RDT antikoerper, RTPS1 antikoerper, SNF5 antikoerper, SNF5L1 antikoerper, Sfh1p antikoerper, Snr1 antikoerper, hSNFS antikoerper, AU020204 antikoerper, Baf47 antikoerper, Ini1 antikoerper, SNF5/INI1 antikoerper, Snf5 antikoerper, fc51b01 antikoerper, smarcb1 antikoerper, wu:fc51b01 antikoerper, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 antikoerper, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 S homeolog antikoerper, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1b antikoerper, SMARCB1 antikoerper, Smarcb1 antikoerper, smarcb1.S antikoerper, smarcb1b antikoerper

Molekulargewicht: 45 kDa

NCBI Accession: NP_001007469