CDT1 Antikörper

Produktnummer ABIN6942498

Produktdetails anti-CDT1 Antikörper

Target: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CDT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Human

Kreuzreaktivität (Details): WB(1:500-1000), IHC-P(1:25-50)

Aufreinigung: Purified by Protein A.

Immunogen: Human CDT1 aa 100 to the C-terminus

Klon: 2H5

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu CDT1

Target: CDT1 (Chromatin Licensing and DNA Replication Factor 1 (CDT1))

Andere Bezeichnung: CDT1 (CDT1 Produkte)

Synonyme: CDT1 antikoerper, fc37h07 antikoerper, im:7158083 antikoerper, wu:fc37h07 antikoerper, wu:fi38h09 antikoerper, xcdt1 antikoerper, DUP antikoerper, RIS2 antikoerper, 2610318F11Rik antikoerper, AW545653 antikoerper, C76791 antikoerper, Ris2 antikoerper, dup antikoerper, ris2 antikoerper, chromatin licensing and DNA replication factor 1 antikoerper, chromatin licensing and DNA replication factor 1 L homeolog antikoerper, CDT1 antikoerper, cdt1 antikoerper, Cdt1 antikoerper, cdt1.L antikoerper

Hintergrund:

Synonyms: DNA replication factor Cdt1, Double parked homolog, DUP, CDT1, chromatin licensing and DNA replication factor 1.

Background: Human Cdt1 is a nuclear localizing replication initiation factor that is expressed only during the G1 and S phases of the cell cycle. In conjunction with Cdc18, Cdt1 is required to load the MCM protein Cdc21 onto chromatin at the end of mitosis which is necessary to initiate DNA replication. After S-phase onset, Cdt1 protein levels decrease and are barely detectable in cells in early S-phase or G2. However, Cdt1 mRNA is expressed in S-phase-arrested cells, and its levels do not change dramatically during the cell cycle, suggesting that proteolytic degradation rather than transcriptional controls ensure proper accumulation of Cdt1. Cdt1 can associate with the DNA replication inhibitor geminin, which is present in the S and G2 phases of the cell cycle. Inhibition of DNA replication by geminin in cell-free DNA replication extracts can be reversed by the addition of excess Cdt1. Geminin may be responsible for preventing inappropriate origin firing by targeting Cdt1.

Gen-ID: 81620

UniProt: Q9H211

Pathways: MAPK Signalweg, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA