Cytochrome C Antikörper (AA 1-80)

Produktnummer ABIN6934821

Produktdetails anti-Cytochrome C Antikörper

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Bindungsspezifität: AA 1-80

Reaktivität: Human, Ratte, Maus, Pferd, Hund, Taube, Drosophila melanogaster, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytochrome C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Spezifität: It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Aufreinigung: Purified by Protein A/G

Immunogen: Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon cytochrome c

Klon: SPM389

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Andere Bezeichnung: CYCS (CYCS Produkte)

Synonyme: CYC antikoerper, HCS antikoerper, THC4 antikoerper, cyct antikoerper, cyc antikoerper, hcs antikoerper, CYCS antikoerper, cytc antikoerper, CYCSA antikoerper, CG17903 antikoerper, Cyt C antikoerper, Cyt c antikoerper, Cyt-c2 antikoerper, CytC-2 antikoerper, Cytc antikoerper, Cytc-p antikoerper, DC4 antikoerper, DMc01 antikoerper, Dmel\\CG17903 antikoerper, cyt c antikoerper, cyt c-p antikoerper, cyt-c-p antikoerper, cyt-c2 antikoerper, cyt.c antikoerper, cytochrome c antikoerper, dc4 antikoerper, pDMc01 antikoerper, wu:fk52b01 antikoerper, zgc:86706 antikoerper, ENSMUSG00000058927 antikoerper, cytochrome c, somatic antikoerper, cytochrome c, somatic S homeolog antikoerper, Cytochrome c proximal antikoerper, cytochrome c, somatic b antikoerper, cytochrome c, somatic pseudogene antikoerper, cytochrome c-like antikoerper, cytochrome c antikoerper, CYCS antikoerper, cycs.S antikoerper, cycs antikoerper, Cycs antikoerper, Cyt-c-p antikoerper, cycsb antikoerper, Gm10053 antikoerper, LOC100724240 antikoerper, CYC antikoerper, LOC100847700 antikoerper, LOC101107954 antikoerper, LOC106829744 antikoerper, LOC106992390 antikoerper, LOC102085712 antikoerper

Molekulargewicht: 15kDa

Gen-ID: 54205

UniProt: P99999

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity