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Myc Tag Antikörper (C-Term)

IF, IHC, FACS, StM Wirt: Maus Monoclonal 9E10-3 unconjugated
Produktnummer ABIN6934473
  • Target Alle Myc Tag Produkte
    Myc Tag
    Bindungsspezifität
    • 21
    • 18
    • 12
    • 8
    • 6
    • 3
    • 2
    • 1
    AA 408-439, C-Term
    Reaktivität
    Bitte anfragen
    Wirt
    • 78
    • 43
    • 6
    • 5
    • 5
    • 3
    • 1
    • 1
    Maus
    Klonalität
    • 80
    • 50
    • 10
    Monoklonal
    Konjugat
    • 64
    • 13
    • 10
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Myc Tag Antikörper ist unkonjugiert
    Applikation
    • 108
    • 51
    • 36
    • 36
    • 25
    • 23
    • 23
    • 18
    • 15
    • 11
    • 11
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
    Spezifität
    It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.
    Keine Kreuzreaktivität
    Huhn, Maus, Ratte (Rattus)
    Aufreinigung
    Purified by Protein A/G
    Immunogen
    A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.
    Klon
    9E10-3
    Isotyp
    IgG1 kappa
  • Applikationshinweise

    Positive Control: HL-60 cells. Cervical Carcinoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Haltbarkeit
    24 months
  • Target
    Myc Tag
    Abstract
    Myc Tag Produkte
    Substanzklasse
    Tag
    Molekulargewicht
    62-64kDa
    Gen-ID
    4609
    UniProt
    P01106
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