Myc Tag Antikörper (C-Term)
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- Target Alle Myc Tag Produkte
- Myc Tag
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Bindungsspezifität
- AA 408-439, C-Term
- Reaktivität
- Bitte anfragen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Myc Tag Antikörper ist unkonjugiert
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Applikation
- Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
- Spezifität
- It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.
- Keine Kreuzreaktivität
- Huhn, Maus, Ratte (Rattus)
- Aufreinigung
- Purified by Protein A/G
- Immunogen
- A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.
- Klon
- 9E10-3
- Isotyp
- IgG1 kappa
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- Applikationshinweise
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Positive Control: HL-60 cells. Cervical Carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Haltbarkeit
- 24 months
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- Target
- Myc Tag
- Abstract
- Myc Tag Produkte
- Substanzklasse
- Tag
- Molekulargewicht
- 62-64kDa
- Gen-ID
- 4609
- UniProt
- P01106
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