COL7A1 Antikörper

Produktnummer ABIN6934193

Produktdetails anti-COL7A1 Antikörper

Target: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Reaktivität: Human, Meerschweinchen, Pferd, Schaf, Rind (Kuh), Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser COL7A1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Staining Methods (StM)

Spezifität: Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human Collagen VII protein

Klon: LH7-2

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.

Known Application: ELISA (For coating, order antibody without BSA), Western Blot (0.5-1.0 μg/mL)Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu COL7A1

Target: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Andere Bezeichnung: COL7A1 (COL7A1 Produkte)

Synonyme: COL7A1 antikoerper, EBD1 antikoerper, EBDCT antikoerper, EBR1 antikoerper, AW209154 antikoerper, collagen type VII alpha 1 chain antikoerper, collagen, type VII, alpha 1 antikoerper, COL7A1 antikoerper, col7a1 antikoerper, Col7a1 antikoerper

Molekulargewicht: 290kDa

Gen-ID: 1294

UniProt: Q02388