COL7A1 Antikörper

Produktnummer ABIN6937011

Produktdetails anti-COL7A1 Antikörper

Target: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser COL7A1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Recognizes an epitope located on collagenase digested Type VII collagen (150 kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Kreuzreaktivität (Details): Human. Sheep. Goat. Horse. Guinea Pig. Cow.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Collagen VII protein

Klon: LH7-2

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL)Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, cells. Human lymphocytes. Human lymph node or tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu COL7A1

Target: COL7A1 (Collagen, Type VII, alpha 1 (COL7A1))

Andere Bezeichnung: COL7A1 (COL7A1 Produkte)

Synonyme: COL7A1 antikoerper, EBD1 antikoerper, EBDCT antikoerper, EBR1 antikoerper, AW209154 antikoerper, collagen type VII alpha 1 chain antikoerper, collagen, type VII, alpha 1 antikoerper, COL7A1 antikoerper, col7a1 antikoerper, Col7a1 antikoerper

Hintergrund: Alpha 1 type VII collagen, Collagen 7, Collagen alpha 1(VII) chain, Collagen type VII alpha 1, Collagen VII alpha 1 polypeptide Col7a1, EBD1, EBDCT, EBR1, Long-chain (LC) collagen,Collagen VII
Cellular localisation: Secreted. Extracellular matrix. Basement Membrane.

Molekulargewicht: 290kDa

Gen-ID: 1294, 476218

UniProt: Q02388