N-Cadherin Antikörper (Intracellular)

Produktnummer ABIN6934768

Produktdetails anti-N-Cadherin Antikörper

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Bindungsspezifität: Intracellular

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser N-Cadherin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. Results aid in the classification of malignant non-carcinomatous neoplasms including mesotheliomas, chordomas, synovial sarcomas, malignant melanomas, epithelioid sarcomas, epithelioid angiosarcomas, clear cell sarcomas as well as serous and endometrioid tumors of the ovary have been demonstrated to be N-cadherin positive, whereas mucinous tumors are negative. Other N-cadherin-positive neoplasms include renal cell carcinomas and some variant breast tumors, including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas.

Kreuzreaktivität (Details): Human, Mouse,

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human CDH2 intracellular domain

Klon: CDH2-1573

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: CDH2 (CDH2 Produkte)

Synonyme: CD325 antikoerper, CDHN antikoerper, CDw325 antikoerper, NCAD antikoerper, N-cadherin antikoerper, cadherin-2 antikoerper, cdhn antikoerper, ncad antikoerper, cdw325 antikoerper, CDH2 antikoerper, Cadherin-2 antikoerper, Ncad antikoerper, glo antikoerper, lyr antikoerper, pac antikoerper, wu:fb47h04 antikoerper, cadherin 2 antikoerper, cadherin 2 S homeolog antikoerper, cadherin 2, type 1, N-cadherin (neuronal) antikoerper, CDH2 antikoerper, cdh2.S antikoerper, cdh2 antikoerper, Cdh2 antikoerper

Hintergrund: Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD,N-Cadherin / Cadherin-2 / CD325 (NCAD)
Cellular localisation: Cell surface

Molekulargewicht: 130-140kDa

Gen-ID: 1000, 464829

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane