SPI1 Antikörper (AA 16-170)

Produktnummer ABIN6938424

Produktdetails anti-SPI1 Antikörper

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Bindungsspezifität: AA 16-170

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SPI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 16-170) of human PU.1 protein (Exact sequence is proprietary)

Klon: PU1-2146

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Western Blot (1-2 μg/mL),Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, K562 or THP-1 cells. Lymph Node, Spleen, Hodgkin s Lymphoma, Colon Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Andere Bezeichnung: SPI1 (SPI1 Produkte)

Synonyme: OF antikoerper, PU.1 antikoerper, SFPI1 antikoerper, SPI-1 antikoerper, SPI-A antikoerper, Dis-1 antikoerper, Dis1 antikoerper, Sfpi-1 antikoerper, Sfpi1 antikoerper, Spi-1 antikoerper, Tcfpu1 antikoerper, Tfpu.1 antikoerper, Pu.1 antikoerper, cb1086 antikoerper, pu.1 antikoerper, pu1 antikoerper, sfpi1 antikoerper, si:by184l24.2 antikoerper, spi1 antikoerper, SPI1/PU.1 antikoerper, spi1l antikoerper, zgc:152991 antikoerper, Spi-1 proto-oncogene antikoerper, spleen focus forming virus (SFFV) proviral integration oncogene antikoerper, Spi-1 proto-oncogene b antikoerper, Spi-1 proto-oncogene a antikoerper, SPI1 antikoerper, Spi1 antikoerper, spi1b antikoerper, spi1a antikoerper

Hintergrund: Transcription Factor spi1, 31 kDa Transforming Protein, Hematopoietic Transcription Factor PU.1, SFPI1, SPI1, SPIA, Spleen focus forming virus (SFFV) proviral integration oncogene spi1,PU.1 (SPI-1) (B-Cell Marker)
Cellular localisation: Nuclear

Molekulargewicht: 40kDa

Gen-ID: 6688, 502511

UniProt: P17947

Pathways: Stem Cell Maintenance