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Myc Tag Antikörper (AA 408-439)

Reaktivität: Human IF, FACS, IHC (f) Wirt: Maus Monoclonal 9E10-3 unconjugated

Produktnummer ABIN6937982

Datenblatt (PDF)

Target Alle Myc Tag Produkte

Myc Tag
Bindungsspezifität
20

18

12

8

6

3

2

1
AA 408-439
Reaktivität
22

5

1
Human
Wirt
77

43

6

5

5

3

1

1
Maus
Passende Sekundärantikörper
Klonalität
79

50

10
Monoklonal
Konjugat
63

13

10

7

4

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Myc Tag Antikörper ist unkonjugiert
Applikation
108

51

36

35

25

23

23

17

15

11

11

6

6

4

4

4

4

3

2

2

1

1

1

1

1

1
Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität

It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

Keine Kreuzreaktivität

Huhn, Maus, Ratte (Rattus)

Kreuzreaktivität (Details)

Human. Does not react with Mouse, Rat or Chicken.

Aufreinigung

1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen

A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.

Klon

9E10-3

Isotyp

IgG1 kappa
anti-Myc Tag antibody
WB, IP, ELISA, IF Wirt: Maus Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
Reaktivität: Human WB, IP, IHC (p), FACS Wirt: Maus Monoclonal 9E10 unconjugated

anti-Myc Tag antibody
WB, IP, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
Reaktivität: Human FACS Wirt: Maus Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
Reaktivität: Human WB, IP, IHC (p), FACS Wirt: Maus Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA, IF Wirt: Maus Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA Wirt: Maus Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF Wirt: Maus Monoclonal 3B8 unconjugated

anti-Myc Tag antibody (Biotin)
WB, IP, ELISA, IHC Wirt: Kaninchen Polyclonal Biotin

anti-Myc Tag (AA 410-419) antibody
WB Wirt: Ziege Polyclonal unconjugated

Applikationshinweise

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: HL-60 cells. Cervical Carcinoma.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1.0 mg/mL

Buffer

Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel

Azide free

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit

24 months
Target

Myc Tag

Abstract

Myc Tag Produkte

Substanzklasse

Tag

Hintergrund

Class E basic helix-loop-helix protein 39 (bHLHe39), MRTL, Myc2, Niard, Nird, Proto-oncogene c-Myc, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog,c-Myc Oncoprotein
Cellular localisation: Nuclear

Molekulargewicht

62-64kDa

Gen-ID

4609, 202453

UniProt

P01106