GAD65 Antikörper (AA 6-99)

Produktnummer ABIN6937293

Produktdetails anti-GAD65 Antikörper

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Bindungsspezifität: AA 6-99

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GAD65 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: This MAb recognizes a protein of 65 kDa, which is identified as glutamic acid decarboxylase 2 (GDA2). It is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. There are two forms of glutamic acid decarboxylases (GAD s) that are found in the brain: GAD2 (also known as GAD65) and GAD1 (also known as GAD67). GAD1 and GAD2 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD2 localizes to synaptic vesicle membranes in nerve terminals, while GAD1 is distributed throughout the cell. A pathogenic role for GAD2 is identified in the human pancreas since it has been identified as an autoantibody and an auto-reactive T cell target in insulin-dependent diabetes.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)

Klon: GAD2-1960

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Pancreas or Brain (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Andere Bezeichnung: GAD2 (GAD2 Produkte)

Synonyme: GAD65 antikoerper, gad65 antikoerper, F12P19.12 antikoerper, F12P19_12 antikoerper, GLUTAMATE DECARBOXYLASE 2 antikoerper, glutamate decarboxylase 2 antikoerper, zgc:112198 antikoerper, 6330404F12Rik antikoerper, GAD(65) antikoerper, Gad-2 antikoerper, glutamate decarboxylase 2 antikoerper, glutamate decarboxylase 1 antikoerper, glutamic acid decarboxylase 2 antikoerper, GAD2 antikoerper, PTRG_06148 antikoerper, LOC9324534 antikoerper, Gad2 antikoerper, GAD1 antikoerper, gad2 antikoerper

Hintergrund: DCE2, GAD2, Glutamic Acid Decarboxylase 2 (Pancreas), Glutamic Acid Decarboxylase 2 (Pancreatic Islets and Brain 65 kDa), Glutamic Acid Decarboxylase 65 (GAD65),GAD2 / GAD65 (GABAergic Neuronal Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 65kDa

Gen-ID: 2572, 231829

UniProt: Q05329