GAD65 Antikörper (N-Term)

Produktnummer ABIN5518755

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GAD65 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Ratte und Maus.Es wurde in 1 Publikation zitiert.

Kurzübersicht für GAD65 Antikörper (N-Term) (ABIN5518755)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GAD65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GAD65 Antikörper

Bindungsspezifität: AA 131-164, N-Term

Verwendungszweck: Anti-GAD65/GAD2 Antibody

Sequenz: KVIDFHYPNE LLQEYNWELA DQPQNLEEIL MHCQ

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-GAD65/GAD2 Antibody (ABIN5518755). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65, different from the related mouse and rat sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-GAD65 Antikörper (ABIN5518755)

Tian, Ma, Shi, Lou, Liu, Li: "Acrylamide neurotoxicity on the cerebrum of weaning rats." in: Neural regeneration research, Vol. 10, Issue 6, pp. 938-43, (2015) (PubMed).

Details zu GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Andere Bezeichnung: GAD2

Hintergrund:

Synonyms: Glutamate decarboxylase 2,4.1.1.15,65 kDa glutamic acid decarboxylase,GAD-65,Glutamate decarboxylase 65 kDa isoform,GAD2,GAD65,

Tissue Specificity: Detected in thymus and in activated splenocytes.

Background: Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Molekulargewicht: 65 kDa

Gen-ID: 2572

UniProt: Q05329