FAT1 Antikörper (Cytoplasmic Domain)

Produktnummer ABIN6937215

Produktdetails anti-FAT1 Antikörper

Target: FAT1 (FAT Tumor Suppressor Homolog 1 (FAT1))

Bindungsspezifität: Cytoplasmic Domain

Reaktivität: Drosophila melanogaster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FAT1 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Coating (Coat), Immunofluorescence (IF)

Spezifität: The FAT proteins are members of the Cadherin superfamily homologous to the Drosophila Fat protein that functions as a positive regulator of planar cell polarity in the Drosophila wing. FAT1 is an unusual cadherin that controls cell growth and planar polarity while acting as a tumor suppressor. FAT1 is a proximal element of a signaling pathway that determines both cellular polarity in the plane of the monolayer and directed actin-dependent cell motility. FAT1 is localized at the leading edge of lamellipodia, filopodia and microspike tips where it directly interacts with Ena/VASP proteins to regulate the actin polymerization complex. When targeted to mitochondrial outer leaflets, the cytoplasmic domain of FAT1 recruits components of the actin polymerization machinery sufficient to induce ectopic actin polymerization. FAT1 expression in vascular smooth muscle cells (VSMCs) increases significantly after arterial injury or growth factor stimulation, implicating FAT1 in the control of VSMC functions central to vascular remodeling by facilitating migration and limiting proliferation. FAT1 is also involved in psychic disorders, and its action may be of patho-physiological importance.

Kreuzreaktivität (Details): Drosophila melanogaster. Expected to cross-react with Chimpanzee. Rat (92 % . 85 % identity with immunogen. respectively) due to sequence homology.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Cytoplasmic domain of Drosophila Fat protein.

Klon: FAT1-3D7-1

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Wild type imaginal discs from third instar Drosophila larvae.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Purified Ab WITHOUT BSA at 1.0mg/ml

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu FAT1

Target: FAT1 (FAT Tumor Suppressor Homolog 1 (FAT1))

Andere Bezeichnung: FAT1 (FAT1 Produkte)

Synonyme: CDHF7 antikoerper, CDHR8 antikoerper, FAT antikoerper, ME5 antikoerper, hFat1 antikoerper, Fath antikoerper, mFat1 antikoerper, AU023433 antikoerper, 2310038E12Rik antikoerper, FAT1 antikoerper, FAT atypical cadherin 1 antikoerper, FAT tumor suppressor homolog 1 (Drosophila) antikoerper, protocadherin Fat 1 antikoerper, FAT1 antikoerper, Fat1 antikoerper, LOC100175143 antikoerper, fat1 antikoerper

Hintergrund: Cadherin family member 7 precursor (CDHF7), Cadherin ME5, Cadherin related tumor suppressor homolog precursor (FAT protein homolog), FAT tumor suppressor homolog 1, hFat 1, Homolog of Drosophila tumor suppressor FAT precursor, nuclear form, Protein fat homolog, Protocadherin Fat 1,FAT1 (FAT atypical cadherin 1)
Cellular localisation: Cell membrane. Nucleus. Cytoplasm > perinuclear region.

Molekulargewicht: 500kDa

Gen-ID: 2195, 481371

UniProt: Q14517