ATP6V0A2 Antikörper (AA 72-121)

Produktnummer ABIN6741595

Produktdetails anti-ATP6V0A2 Antikörper

Target: ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))

Bindungsspezifität: AA 72-121

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP6V0A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human ATP6V0A2

Homologie: Percent identity by BLAST analysis: Human, Rat (100%) Dog, Horse (92%) Pig (91%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human ATP6V0A2 (Q9Y487, NP_036595). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat (100%), Monkey, Galago, Elephant, Dog, Bat, Horse (92%), Pig (91%), Bovine, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ATP6V0A2

Target: ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))

Andere Bezeichnung: ATP6V0A2 (ATP6V0A2 Produkte)

Synonyme: A2 antikoerper, ARCL antikoerper, ARCL2A antikoerper, ATP6A2 antikoerper, ATP6N1D antikoerper, J6B7 antikoerper, RTF antikoerper, STV1 antikoerper, TJ6 antikoerper, TJ6M antikoerper, TJ6S antikoerper, VPH1 antikoerper, WSS antikoerper, 8430408C20Rik antikoerper, AI385560 antikoerper, ATP6a2 antikoerper, AW489264 antikoerper, Atp6n1d antikoerper, Atp6n2 antikoerper, C76904 antikoerper, ISF antikoerper, SHIF antikoerper, Stv1 antikoerper, TJ6s antikoerper, Tj6 antikoerper, V-ATPase 116 kDa antikoerper, V-ATPase a2 antikoerper, Cc1-3 antikoerper, J6b7 antikoerper, atp6v0a2 antikoerper, si:ch211-199i18.4 antikoerper, si:ch211-106a19.2 antikoerper, ATPase H+ transporting V0 subunit a2 antikoerper, ATPase, H+ transporting, lysosomal V0 subunit A2 antikoerper, ATPase, H+ transporting, lysosomal V0 subunit a2a antikoerper, ATPase, H+ transporting, lysosomal V0 subunit a2b antikoerper, ATP6V0A2 antikoerper, Atp6v0a2 antikoerper, atp6v0a2a antikoerper, atp6v0a2b antikoerper

Hintergrund: Name/Gene ID: ATP6V0A2
Subfamily: ATPase - V type
Family: Transporter

Synonyms: ATP6V0A2, A2V-ATPase, ARCL, ARCL2A, ATP6A2, ATP6N1D, RTF, STV1, V-ATPase 116 kDa, TJ6S, WSS, J6B7, TJ6, TJ6M, V-ATPase 116 kDa isoform a2, VPH1

Gen-ID: 23545

NCBI Accession: NP_036595

UniProt: Q9Y487

Pathways: Transition Metal Ion Homeostasis, Proton Transport