NR0B1 Antikörper (AA 359-408)

Produktnummer ABIN462167

Produktdetails anti-NR0B1 Antikörper

Target: NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))

Bindungsspezifität: AA 359-408

Reaktivität: Human, Maus, Ratte, Hund, Pferd, Rind (Kuh), Meerschweinchen, Kaninchen, Affe, Zebrafisch (Danio rerio), Huhn, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NR0B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: Human NR0B1 / DAX-1

Kreuzreaktivität: Huhn, Hund, Maus, Schwein, Ratte (Rattus)

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Zebrafish (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa359-408 of human NR0B1 (Q9BG96, NP_000466). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Chicken, Platypus, Lizard (100%), Galago, Hamster, Xenopus, Seabass, Salmon, Stickleback, Medaka (92%), Bat (91%), Zebrafish (85%), Catfish (81%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 2 % sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu NR0B1

Target: NR0B1 (Nuclear Receptor Subfamily 0, Group B, Member 1 (NR0B1))

Andere Bezeichnung: NR0B1 / DAX1 (NR0B1 Produkte)

Synonyme: DAX antikoerper, NR0B1 antikoerper, dax1 antikoerper, AHC antikoerper, AHCH antikoerper, AHX antikoerper, DAX-1 antikoerper, DAX1 antikoerper, DSS antikoerper, GTD antikoerper, HHG antikoerper, NROB1 antikoerper, SRXY2 antikoerper, Ahc antikoerper, Ahch antikoerper, Dax1 antikoerper, nuclear receptor subfamily 0 group B member 1 antikoerper, nuclear receptor subfamily 0, group B, member 1 antikoerper, nuclear receptor subfamily 0 group B member 1 L homeolog antikoerper, NR0B1 antikoerper, nr0b1 antikoerper, nr0b1.L antikoerper, Nr0b1 antikoerper

Hintergrund: Name/Gene ID: NR0B1
Subfamily: NR0 Knirps-like
Family: NHR

Synonyms: NR0B1, AHX, AHC, AHCH, DAX1, DSS, DAX 1, DAX-1, Adrenal hypoplasia congenita, Dosage-sensitive sex reversal, GTD, HHG, Nuclear hormone receptor, NROB1, Nuclear receptor DAX-1, SRXY2, Nuclear receptor DAX1, Dax-1 receptor

Gen-ID: 190

NCBI Accession: NP_000466

UniProt: P51843

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling