POLR2B Antikörper (AA 805-854)
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- Target Alle POLR2B Antikörper anzeigen
- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
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Bindungsspezifität
- AA 805-854
- Reaktivität
- Human, Maus, Ratte, Hund, Rind (Kuh), Kaninchen, Meerschweinchen, Pferd, Schwein, Xenopus laevis, Affe
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser POLR2B Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human POLR2B / RPB2
- Homologie
- Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Xenopus (100%) Chicken, Zebrafish (92%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa805-854 of human POLR2B (P30876, NP_000929). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%), Zebra finch, Chicken, Salmon, Stickleback, Zebrafish (92%).
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
- Top Product
- Discover our top product POLR2B Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- After adding water, will consist of PBS buffer with 2 % sucrose
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))
- Andere Bezeichnung
- POLR2B / RPB2 (POLR2B Produkte)
- Synonyme
- Pol2rb antikoerper, Rpb140 antikoerper, Rpb2 antikoerper, POL2RB antikoerper, RPB2 antikoerper, hRPB140 antikoerper, hsRPB2 antikoerper, RNA polymerase II subunit B antikoerper, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa antikoerper, DNA-directed RNA polymerase II subunit RPB2 antikoerper, polymerase (RNA) II (DNA directed) polypeptide B antikoerper, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog antikoerper, POLR2B antikoerper, polr2b antikoerper, LOC100640211 antikoerper, Polr2b antikoerper, polr2b.L antikoerper, rpb-2 antikoerper
- Hintergrund
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Name/Gene ID: POLR2B
Synonyms: POLR2B, HsRPB2, POL2RB, RNA polymerase II subunit B2, RNA polymerase II subunit 2, RPB2, HRPB140 - Gen-ID
- 5431
- NCBI Accession
- NP_000929
- UniProt
- P30876
- Pathways
- Regulatorische RNA Pathways, DNA Reparatur
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