POLR2B Antikörper (AA 805-854)

Produktnummer ABIN6736747

Produktdetails anti-POLR2B Antikörper

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Bindungsspezifität: AA 805-854

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Kaninchen, Meerschweinchen, Pferd, Schwein, Xenopus laevis, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human POLR2B / RPB2

Homologie: Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Xenopus (100%) Chicken, Zebrafish (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa805-854 of human POLR2B (P30876, NP_000929). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Xenopus (100%), Zebra finch, Chicken, Salmon, Stickleback, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Andere Bezeichnung: POLR2B / RPB2 (POLR2B Produkte)

Synonyme: Pol2rb antikoerper, Rpb140 antikoerper, Rpb2 antikoerper, POL2RB antikoerper, RPB2 antikoerper, hRPB140 antikoerper, hsRPB2 antikoerper, RNA polymerase II subunit B antikoerper, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa antikoerper, DNA-directed RNA polymerase II subunit RPB2 antikoerper, polymerase (RNA) II (DNA directed) polypeptide B antikoerper, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog antikoerper, POLR2B antikoerper, polr2b antikoerper, LOC100640211 antikoerper, Polr2b antikoerper, polr2b.L antikoerper, rpb-2 antikoerper

Hintergrund: Name/Gene ID: POLR2B

Synonyms: POLR2B, HsRPB2, POL2RB, RNA polymerase II subunit B2, RNA polymerase II subunit 2, RPB2, HRPB140

Gen-ID: 5431

NCBI Accession: NP_000929

UniProt: P30876

Pathways: Regulatorische RNA Pathways, DNA Reparatur