Fibrillarin Antikörper

Produktnummer ABIN6658299

Produktdetails anti-Fibrillarin Antikörper

Target: Fibrillarin (FBL)

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibrillarin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Fluorescence Microscopy (FM)

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: Anti-Fibrillarin Antibody is directed against yeast Fibrillarin/Nop1p protein. Fibrillarin antibodies are protein G purified from concentrated clarified cell culture supernate. Reactivity is expected against human, mouse, and rat. Cross-reactivity toward other sources has not be determined. Anti-Fibrillarin antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.

Immunogen:

Immunogen: Anti-Fibrillarin Monoclonal Antibody was produced in mouse by repeated immunizations with native yeast nuclear protein preparations.

Immunogen Type: Native Protein

Klon: 38F3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Fibrillarin (Mouse) Antibody is suitable for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band size of approximately 34 kDa in size corresponding to the Fibrillarin/Nop1p proteins.

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Details zu Fibrillarin

Target: Fibrillarin (FBL)

Andere Bezeichnung: Fibrillarin (FBL Produkte)

Synonyme: FIB antikoerper, FLRN antikoerper, RNU3IP1 antikoerper, wu:fb37g12 antikoerper, zgc:56145 antikoerper, zgc:77130 antikoerper, FBL antikoerper, AL022665 antikoerper, CG9888 antikoerper, Dmel\\CG9888 antikoerper, Fibri antikoerper, GCR-6 antikoerper, GCR6 antikoerper, Pen59C5 antikoerper, fib antikoerper, pen59C5 antikoerper, MGC76139 antikoerper, fbl antikoerper, fibrillarin antikoerper, Fibrillarin antikoerper, putative fibrillarin antikoerper, fibrillarin S homeolog antikoerper, FBL antikoerper, fbl antikoerper, Fbl antikoerper, Fib antikoerper, fib1 antikoerper, LMJF_19_0100 antikoerper, fbl.S antikoerper

Hintergrund:

Synonyms: mouse anti-Fibrillarin Antibody, rRNA 2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1

Background: Fibrillarin Antibody detects Nop1p which was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. Anti-Fibrillarin Antibody is ideal for investigators involved in Cell Proliferation and Cancer Research.

Gene Name: NOP1

Gen-ID: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process